Title:
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Role of the pre-neck appendage protein (DpoSep7) from phage vB_SepiS-phiIPLA7 in biofilm disruption of staphylococcal species
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Author:
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Gutiérrez, Diana; Briers, Yves; Rodríguez Rubio, Lorena; Martínez, Beatriz; Rodríguez, Ana; Lavigne, Rob; García, Pilar
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Other authors:
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Universitat de Barcelona |
Abstract:
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Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 μM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials. |
Subject(s):
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-Estafilococs -Staphylococcus |
Rights:
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cc-by (c) Gutiérrez, Diana et al., 2015
http://creativecommons.org/licenses/by/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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Frontiers Media
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