Title:
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Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci
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Author:
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Rodriguez Rubio, Lorena; Chang, Wai-Ling; Gutiérrez, Diana; Lavigne, Rob; Martínez, Beatriz; Rodríguez, Ana; Govers, Sander K.; Aertsen, Abram; Hirl, Christine; Biebl, Manfred; Briers, Yves; García, Pilar
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Other authors:
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Universitat de Barcelona |
Abstract:
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Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10min. In addition, lower doses of Art240 are required to achieve the same bactericidal effect. |
Subject(s):
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-Estreptococs -Enzims microbians -Bacteriòfags -Streptococcus -Microbial enzymes -Bacteriophages |
Rights:
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cc-by (c) Rodriguez Rubio, Lorena et al., 2016
http://creativecommons.org/licenses/by/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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Nature Publishing Group
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