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| dc.contributor.author | Vergara, Andrea |
|---|---|
| dc.contributor.author | Boutal, Hervé |
| dc.contributor.author | Ceccato, Adrian |
| dc.contributor.author | López, Miriam |
| dc.contributor.author | Cruells, Adrián |
| dc.contributor.author | Bueno, Leticia |
| dc.contributor.author | Moreno-Morales, Javier |
| dc.contributor.author | Puig de la Bellacasa, Jordi |
| dc.contributor.author | Castro, Pedro |
| dc.contributor.author | Torres Martí, Antoni |
| dc.contributor.author | Marco Reverté, Francesc |
| dc.contributor.author | Casals Pascual, Climent |
| dc.contributor.author | Vila Estapé, Jordi |
| dc.date | 2020-02-21T12:16:25Z |
| dc.date | 2020-02-21T12:16:25Z |
| dc.date | 2020-01-11 |
| dc.date | 2020-02-21T12:16:25Z |
| dc.identifier | 2076-2607 |
| dc.identifier | http://hdl.handle.net/2445/150965 |
| dc.identifier | 695192 |
| dc.identifier.uri | http://hdl.handle.net/2445/150965 |
| dc.description | Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allowan earlier administration of a more appropriate antibiotic and could improve the outcome of thesepatients. The aim of this study was to develop a rapid protocol to identify the main microorganismsinvolved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples.First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL),endotracheal aspirate (EA) or bronchoaspirate (BAS) was set up. A specific LAMP forStaphylococcusaureus,Escherichia coli,Klebsiella pneumoniae, Pseudomonas aeruginosa,Stenotrophomonas maltophiliaandAcinetobacter baumanniiwas performed with the extracted solution at 65◦C for 30-40 min. Overall,58 positive BAL and 83 EA/BAS samples were tested. The limits of detection varied according to themicroorganism detected. Validation of the LAMP assay with BAL samples showed that the assay was 100% specific and 86.3% sensitive (positive predictive value of 100% and a negative predictive valueof 50%) compared with culture. Meanwhile for BAS/EA samples, the assay rendered the followingstatistical parameters: 100% specificity, 94.6% sensitivity, 100% positive predictive value and 69.2%negative predictive value. The turnaround time including sample preparation and LAMP was circa1 h. LAMP method may be used to detect the most frequent bacteria causing HAP. It is a simple,cheap, sensitive, specific and rapid assay. |
| dc.format | 10 p. |
| dc.format | application/pdf |
| dc.language | eng |
| dc.publisher | MDPI |
| dc.relation | Reproducció del document publicat a: https://doi.org/10.3390/microorganisms8010103 |
| dc.relation | Microorganisms, 2020, vol. 8, num. 1, p. 103 |
| dc.relation | https://doi.org/10.3390/microorganisms8010103 |
| dc.rights | cc-by (c) Vergara, Andrea et al., 2020 |
| dc.rights | http://creativecommons.org/licenses/by/3.0/es |
| dc.rights | info:eu-repo/semantics/openAccess |
| dc.subject | Diagnòstic |
| dc.subject | Medicina intensiva |
| dc.subject | Aparell respiratori |
| dc.subject | Pneumònia adquirida a la comunitat |
| dc.subject | Diagnosis |
| dc.subject | Critical care medicine |
| dc.subject | Respiratory organs |
| dc.subject | Community-acquired pneumonia |
| dc.title | Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia |
| dc.type | info:eu-repo/semantics/article |
| dc.type | info:eu-repo/semantics/publishedVersion |
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