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Títol:	Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia
Autor/a:	Vergara, Andrea; Boutal, Hervé; Ceccato, Adrian; López, Miriam; Cruells, Adrián; Bueno, Leticia; Moreno-Morales, Javier; Puig de la Bellacasa, Jordi; Castro, Pedro; Torres Martí, Antoni; Marco Reverté, Francesc; Casals Pascual, Climent; Vila Estapé, Jordi
Notes:	Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allowan earlier administration of a more appropriate antibiotic and could improve the outcome of thesepatients. The aim of this study was to develop a rapid protocol to identify the main microorganismsinvolved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples.First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL),endotracheal aspirate (EA) or bronchoaspirate (BAS) was set up. A specific LAMP forStaphylococcusaureus,Escherichia coli,Klebsiella pneumoniae, Pseudomonas aeruginosa,Stenotrophomonas maltophiliaandAcinetobacter baumanniiwas performed with the extracted solution at 65◦C for 30-40 min. Overall,58 positive BAL and 83 EA/BAS samples were tested. The limits of detection varied according to themicroorganism detected. Validation of the LAMP assay with BAL samples showed that the assay was 100% specific and 86.3% sensitive (positive predictive value of 100% and a negative predictive valueof 50%) compared with culture. Meanwhile for BAS/EA samples, the assay rendered the followingstatistical parameters: 100% specificity, 94.6% sensitivity, 100% positive predictive value and 69.2%negative predictive value. The turnaround time including sample preparation and LAMP was circa1 h. LAMP method may be used to detect the most frequent bacteria causing HAP. It is a simple,cheap, sensitive, specific and rapid assay.
Matèries:	-Diagnòstic -Medicina intensiva -Aparell respiratori -Pneumònia adquirida a la comunitat -Diagnosis -Critical care medicine -Respiratory organs -Community-acquired pneumonia
Drets:	cc-by (c) Vergara, Andrea et al., 2020 http://creativecommons.org/licenses/by/3.0/es
Tipus de document:	Article Article - Versió publicada
Publicat per:	MDPI
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