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Title:	An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays
Author:	Höftberger, Romana; Sabater, Lidia; Marignier, Romain; Aboul-Enein, Fahmy; Bernard-Valnet, Raphaël; Rauschka, Helmut; Ruiz, Anne; Blanco Morgado, Yolanda; Graus Ribas, Francesc; Dalmau Obrador, Josep; Saiz Hinajeros, Albert
Notes:	Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection.
Subject(s):	-Immunohistoquímica -Immunoglobulines -Immunohistochemistry -Immunoglobulins
Rights:	cc-by (c) Höftberger, Romana et al., 2013 http://creativecommons.org/licenses/by/3.0/es
Document type:	Article Article - Published version
Published by:	Public Library of Science (PLoS)
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