Título:
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An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays
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Autor/a:
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Höftberger, Romana; Sabater, Lidia; Marignier, Romain; Aboul-Enein, Fahmy; Bernard-Valnet, Raphaël; Rauschka, Helmut; Ruiz, Anne; Blanco Morgado, Yolanda; Graus Ribas, Francesc; Dalmau Obrador, Josep; Saiz Hinajeros, Albert
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Otros autores:
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Universitat de Barcelona |
Abstract:
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Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection. |
Materia(s):
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-Immunohistoquímica -Immunoglobulines -Immunohistochemistry -Immunoglobulins |
Derechos:
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cc-by (c) Höftberger, Romana et al., 2013
http://creativecommons.org/licenses/by/3.0/es |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Public Library of Science (PLoS)
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