dc.contributor.author |
Calvo Lozano, Olalla |
dc.contributor.author |
Sierra, Miquel |
dc.contributor.author |
Soler Aznar, Maria |
dc.contributor.author |
Estévez, M. Carmen |
dc.contributor.author |
Chiscano-Camón, Luis |
dc.contributor.author |
Ruiz-Sanmartin, Adolfo |
dc.contributor.author |
Ruiz-Rodriguez, Juan Carlos |
dc.contributor.author |
Ferrer, Ricard |
dc.contributor.author |
González-López, Juanjo |
dc.contributor.author |
Esperalba, Juliana |
dc.contributor.author |
Fernandez-Naval, Candela |
dc.contributor.author |
Bueno, Leticia |
dc.contributor.author |
López-Aladid, Ruben |
dc.contributor.author |
Torres, Antoni |
dc.contributor.author |
Fernández-Barat, Laia |
dc.contributor.author |
Attoumani, Sarah |
dc.contributor.author |
Charrel, Rémi |
dc.contributor.author |
Coutard, Bruno |
dc.contributor.author |
Lechuga, Laura M |
dc.date |
2022 |
dc.identifier |
https://ddd.uab.cat/record/250892 |
dc.identifier |
urn:10.1021/acs.analchem.1c03850 |
dc.identifier |
urn:oai:ddd.uab.cat:250892 |
dc.identifier |
urn:pmcid:PMC8751014 |
dc.identifier |
urn:pmc-uid:8751014 |
dc.identifier |
urn:pmid:34971311 |
dc.identifier |
urn:articleid:15206882v94p975 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:8751014 |
dc.identifier |
urn:icn2uab:6543225 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
European Commission 101003544 |
dc.relation |
Ministerio de Ciencia e Innovación SEV-2017-0706 |
dc.relation |
Ministerio de Economía, Industria y Competitividad BES-2017-080527 |
dc.relation |
Ministerio de Economía y Competitividad TEC 2016-78515-R |
dc.relation |
European Commission 871029 |
dc.relation |
European Commission 644956 |
dc.relation |
Ministerio de Economía y Competitividad RD16/0016/0003 |
dc.relation |
Analytical chemistry ; Vol. 94, Issue 2 (January 2022), p. 975-984 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Immunology |
dc.subject |
Sensors |
dc.subject |
Peptides and proteins |
dc.subject |
Assays |
dc.subject |
Biopolymers |
dc.title |
Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology : Implementation and Clinical Validation |
dc.type |
Article |
dc.description.abstract |
Serological tests are essential for the control and management of COVID-19 pandemic (diagnostics and surveillance, and epidemiological and immunity studies). We introduce a direct serological biosensor assay employing proprietary technology based on plasmonics, which offers rapid (<15 min) identification and quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies in clinical samples, without signal amplification. The portable plasmonic device employs a custom-designed multiantigen (RBD peptide and N protein) sensor biochip and reaches detection limits in the low ng mL -1 range employing polyclonal antibodies. It has also been implemented employing the WHO-approved anti-SARS-CoV-2 immunoglobulin standard. A clinical validation with COVID-19 positive and negative samples (n = 120) demonstrates its excellent diagnostic sensitivity (99%) and specificity (100%). This positions our biosensor as an accurate and easy-to-use diagnostics tool for rapid and reliable COVID-19 serology to be employed both at laboratory and decentralized settings for the disease management and for the evaluation of immunological status during vaccination or treatment. |