Título:
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Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology : Implementation and Clinical Validation
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Autor/a:
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Calvo Lozano, Olalla; Sierra, Miquel; Soler Aznar, Maria; Estévez, M. Carmen; Chiscano-Camón, Luis; Ruiz-Sanmartin, Adolfo; Ruiz-Rodriguez, Juan Carlos; Ferrer, Ricard; González-López, Juanjo; Esperalba, Juliana; Fernandez-Naval, Candela; Bueno, Leticia; López-Aladid, Ruben; Torres, Antoni; Fernández-Barat, Laia; Attoumani, Sarah; Charrel, Rémi; Coutard, Bruno; Lechuga, Laura M
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Abstract:
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Serological tests are essential for the control and management of COVID-19 pandemic (diagnostics and surveillance, and epidemiological and immunity studies). We introduce a direct serological biosensor assay employing proprietary technology based on plasmonics, which offers rapid (<15 min) identification and quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies in clinical samples, without signal amplification. The portable plasmonic device employs a custom-designed multiantigen (RBD peptide and N protein) sensor biochip and reaches detection limits in the low ng mL -1 range employing polyclonal antibodies. It has also been implemented employing the WHO-approved anti-SARS-CoV-2 immunoglobulin standard. A clinical validation with COVID-19 positive and negative samples (n = 120) demonstrates its excellent diagnostic sensitivity (99%) and specificity (100%). This positions our biosensor as an accurate and easy-to-use diagnostics tool for rapid and reliable COVID-19 serology to be employed both at laboratory and decentralized settings for the disease management and for the evaluation of immunological status during vaccination or treatment. |
Materia(s):
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-Immunology -Sensors -Peptides and proteins -Assays -Biopolymers |
Derechos:
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open access
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Tipo de documento:
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Article |
Editor:
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Uri:
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https://ddd.uab.cat/record/250892
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