Title:
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Label-free Bacteria Quantification in Blood Plasma by a Bioprinted Microarray Based Interferometric Point-of-Care Device
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Author:
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Dey, Priyanka; Fabri Faja, Nuria; Calvo Lozano, Olalla; Terborg, Roland A.; Belushkin, Alexander; Yesilköy, Filiz; Fàbrega-Santamaria, Anna; Ruiz Rodríguez, Juan Carlos; Ferrer, Ricard; González López, Juan José; Estévez, M. Carmen; Altug, Hatice; Pruneri, Valerio; Lechuga, Laura M
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Abstract:
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Existing clinical methods for bacteria detection lack speed, sensitivity, and, importantly, point-of-care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Here we report a portable PoC device based on lens-free interferometric microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample-to-data turnaround time of 40 min. Our technology features detection sensitivity of a single bacterial cell both in buffer and in diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and noninfectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality. |
Subject(s):
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-Bacteria -Sepsis -Microarray -Label-free detection -Nanoplasmonics -Plasma samples |
Rights:
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open access
Tots els drets reservats.
https://rightsstatements.org/vocab/InC/1.0/ |
Document type:
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Article |
Published by:
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Share:
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Uri:
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https://ddd.uab.cat/record/218093
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