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Title:	Actin-binding protein α-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor
Author:	Cabello, Nuria; Remelli, Rosaria; Canela, Laia; Soriguera, Ana; Mallol Montero, Josefa; Canela Campos, Enric I.; Robbins, Melanie J.; Lluís i Biset, Carme; Franco Fernández, Rafael; McIlhinney, R. A. Jeffrey; Ciruela Alférez, Francisco
Other authors:	Universitat de Barcelona
Abstract:	Receptors for neurotransmitters require scaffolding proteins for membrane microdomain targeting and for regulating receptor function. Using a yeast two-hybrid screen, α-actinin-1, a major F-actin cross-linking protein, was identified as a binding partner for the C-terminal domain of metabotropic glutamate receptor type 5b (mGlu5b receptor). Co-expression, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between mGlu5b receptor and α-actinin-1 in both transfected HEK-293 cells and rat striatum. The interaction of α-actinin-1 with mGlu5b receptor modulated the cell surface expression of the receptor. This was dependent on the binding of α-actinin-1 to the actin cytoskeleton. In addition, the α-actinin-1/mGlu5b receptor interaction regulated receptor-mediated activation of the mitogen-activated protein kinase pathway. Together, these findings indicate that there is an α-actinin-1-dependent mGlu5b receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning.
Subject(s):	-Àcid glutàmic -Neurotransmissió -Glutamic acid -Neural transmission
Rights:	(c) American Society for Biochemistry and Molecular Biology, 2007
Document type:	Article Article - Published version
Published by:	American Society for Biochemistry and Molecular Biology
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