Title:
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Metabotropic glutamate 1α and adenosine A1 receptors assemble into functionally interacting complexes
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Author:
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Ciruela Alférez, Francisco; Escriche, Marisol; Burgueño, Javier; Angulo, Ester; Casadó, Vicent; Soloviev, Mikhail M.; Canela Campos, Enric I.; Mallol Montero, Josefa; Chan, Wai-Yee; Lluís i Biset, Carme; McIlhinney, R. A. Jeffrey; Franco Fernández, Rafael
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Other authors:
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Universitat de Barcelona |
Abstract:
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Recently, evidence has emerged that seven transmembrane G protein-coupled receptors may be present as homo- and heteromers in the plasma membrane. Here we describe a new molecular and functional interaction between two functionally unrelated types of G protein-coupled receptors, namely the metabotropic glutamate type 1alpha (mGlu(1alpha) receptor) and the adenosine A1 receptors in cerebellum, primary cortical neurons, and heterologous transfected cells. Co-immunoprecipitation experiments showed a close and subtype-specific interaction between mGlu(1alpha) and A1 receptors in both rat cerebellar synaptosomes and co-transfected HEK-293 cells. By using transiently transfected HEK-293 cells a synergy between mGlu(1alpha) and A1 receptors in receptor-evoked [Ca(2+)](i) signaling has been shown. In primary cultures of cortical neurons we observed a high degree of co-localization of the two receptors, and excitotoxicity experiments in these cultures also indicate that mGlu(1alpha) and A1 receptors are functionally related. Our results provide a molecular basis for adenosine/glutamate receptors cross-talk and open new perspectives for the development of novel agents to treat neuropsychiatric disorders in which abnormal glutamatergic neurotransmission is involved. |
Subject(s):
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-Adenosina -Neurotransmissió -Àcid glutàmic -Adenosine -Neural transmission -Glutamic acid |
Rights:
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(c) American Society for Biochemistry and Molecular Biology, 2001
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Document type:
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Article Article - Published version |
Published by:
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American Society for Biochemistry and Molecular Biology
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