To access the full text documents, please follow this link: http://hdl.handle.net/2445/25563

Thyroid hormone regulates reelin and dab1 expression during brain development
Alvarez-Dolado, Manuel; Ruiz, Mónica; Río Fernández, José Antonio del; Alcántara Horrillo, Soledad; Burgaya i Márquez, Ferran; Sheldon, Michael; Nakajima, Kazunori; Bernal, Juan; Howell, Brian W.; Curran, Tom
Universitat de Barcelona
The reelin and dab1 genes are necessary for appropriate neuronal migration and lamination during brain development. Since these processes are controlled by thyroid hormone, we studied the effect of thyroid hormone deprivation and administration on the expression of reelin anddab1. As shown by Northern analysis, in situ hybridization, and immunohistochemistry studies, hypothyroid rats expressed decreased levels of reelinRNA and protein during the perinatal period [embryonic day 18 (E18) and postnatal day 0 (P0)]. The effect was evident in Cajal-Retzius cells of cortex layer I, as well as in layers V/VI, hippocampus, and granular neurons of the cerebellum. At later ages, however, Reelin was more abundant in the cortex, hippocampus, cerebellum, and olfactory bulb of hypothyroid rats (P5), and no differences were detected at P15. Conversely, Dab1 levels were higher at P0, and lower at P5 in hypothyroid animals. In line with these results, reelin RNA and protein levels were higher in cultured hippocampal slices from P0 control rats compared to those from hypothyroid animals. Significantly, thyroid-dependent regulation of reelin anddab1 was confirmed in vivo and in vitro by hormone treatment of hypothyroid rats and organotypic cultures, respectively. In both cases, thyroid hormone led to an increase in reelin expression. Our data suggest that the effects of thyroid hormone on neuronal migration may be in part mediated through the control of reelin anddab1 expression during brain ontogenesis.
Teixit nerviós
Hormones tiroides
Cervell
Nerve tissue
Thyroid hormones
Brain
(c) Society for Neuroscience, 1999
Article
Society for Neuroscience
         

Show full item record

Related documents

Other documents of the same author

Borrell Franco, Víctor; Río Fernández, José Antonio del; Alcántara Horrillo, Soledad; Derer, Michèle; Martinez García, Albert; D'Arcangelo, Gabriella; Nakajima, Kazunori; Mikoshiba, Katsuhiko; Derer, Paul; Curran, Tom
Alcántara Horrillo, Soledad; Ruiz, Mónica; D'Arcangelo, Gabriella; Ezan, Frederic; Lecea Flores de Lemus, Luis de; Curran, Tom; Sotelo, Constantino
Alcántara Horrillo, Soledad; Frisén, Jonás; Río Fernández, José Antonio del; Soriano García, Eduardo; Barbacid, Mariano; Silos-Santiago, Inmaculada
Lecea Flores de Lemus, Luis de; Río Fernández, José Antonio del; Alcántara Horrillo, Soledad; Criado, José R.; Morales, Marisela; Danielson, Patria E.; Henriksen, Steven J.; Soriano García, Eduardo; Sutcliffe, J. Gregor
Martínez García, Albert; Alcántara Horrillo, Soledad; Borrell Franco, Víctor; Río Fernández, José Antonio del; Blasi Cabús, Joan; Otal Agudo, Raquel; Campos Martínez, Narciso; Boronat i Margosa, Albert; Barbacid, Mariano; Silos-Santiago, Inmaculada; Soriano García, Eduardo
 

Coordination

 

Supporters