dc.contributor.author
Blasco, Lucía
dc.contributor.author
Veiga Crespo, Patricia
dc.contributor.author
Viñas, Miquel
dc.contributor.author
Villa, Tomás G.
dc.date.issued
2014-03-21T13:18:31Z
dc.date.issued
2014-03-21T13:18:31Z
dc.date.issued
2011-12-01
dc.date.issued
2014-03-21T13:18:31Z
dc.identifier
https://hdl.handle.net/2445/52756
dc.description.abstract
Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.
dc.format
application/pdf
dc.publisher
Spanish Society for Microbiology (SEM) and Viguera Editores SL
dc.relation
Reproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.149
dc.relation
International Microbiology, 2011, vol. 14, p. 201-206
dc.relation
http://dx.doi.org/10.2436/20.1501.01.149
dc.rights
cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
dc.rights
http://creativecommons.org/licenses/by-nc-sa/3.0/es
dc.rights
info:eu-repo/semantics/openAccess
dc.source
Articles publicats en revistes (Patologia i Terapèutica Experimental)
dc.subject
Saccharomyces cerevisiae
dc.subject
Saccharomyces cerevisiae
dc.title
A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
