Immunostaining Protocol: P-Stat3 (Xenograft and Mice)

We sought to understand the mechanisms behind the potent effect of stromal TGF-beta program on the capacity of colorectal cancer (CRC) cells to initiate metastasis. We discovered that mice subcutaneous tumors and metastases generated in the context of a TGF-beta activated microenvironment displayed prominent accumulation of p-STAT3 in CRC cells compared with those derived from control cells. STAT3 signaling depended on GP130 as shown by strong reduction of epithelial p STAT3 levels upon GP130 shRNA-mediated knockdown in CRC cells.

Document Type

Article

Published version

Language

English

Subjects and keywords

Càncer colorectal; Metàstasi; Expressió gènica; Colorectal cancer; Metastasis; Gene expression

Related items

Reproducció del document publicat a: https://doi.org/10.21769/BioProtoc.1120

Bio-protocol, 2014, vol. 4, num. 9, p. e1120

https://doi.org/10.21769/BioProtoc.1120

Recommended citation

This citation was generated automatically.

Export

DIDL MARC MARC_CCUC METS OAI_DC ORE QDC RDF

Rights

This item appears in the following Collection(s)

Institut de Recerca Biomèdica (IRB Barcelona) [418]

ISGlobal - Institut de Salut Global de Barcelona [61249]

Immunostaining Protocol: P-Stat3 (Xenograft and Mice)

Author

Publication date

Share

Abstract