Bioquímica i Biomedicina Molecular: Enviaments recents
Ara mostrant els elements 21-40 de 918
Isoprenoid biosynthesis via the methylerythritol phosphate pathway. (E)-4-Hydroxy-3-methylbut-2-enyl diphosphate: chemical synthesis and formation from methylerythritol cyclodiphosphate by a cell-free system from Escherichia coli
2-C-Methyl-d-erythritol cyclodiphosphate is converted into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate by a cell-free system from an Escherichia coli strain overexpressing the gcpE gene. The latter diphosphate, representing ...
Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate: a novel system for the genetic analysis of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
Isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP) constitute the basic building block of isoprenoids, a family of compounds that is extraordinarily diverse in structure and function. IPP and ...
Release of active cytokinin by a β-glucosidase localized to the maize root meristem
A β-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts ...
Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
Plant isoprenoids represent a heterogeneous group of compounds which play essential roles not only in growth and development, but also in the interaction of plants with their environment. Higher plants contain two pathways ...
Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative ...
Human gamma-delta T cells are activated by intermediates of the the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis
Activation of Vγ9/Vδ2 T cells by small nonprotein Ags is frequently observed after infection with various viruses, bacteria, and eukaryotic parasites. We suggested earlier that compounds synthesized by the 2-C-methyl-d-erythritol ...
Deuterium-labelled isotopomers of 2-C-methyl-D-erythritol as tools for the elucidation of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
Escherichia coli synthesizes its isoprenoids via the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. The MC4100dxs::CAT strain, defective in deoxyxylulose-5-phosphate synthase, which is the first ...
Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli
An alternative mevalonate-independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli) and plant plastids, although it is not fully elucidated yet. In this work, ...
Characterization of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis
Thiolases are ubiquitous enzymes involved in many essential biochemical processes. Biosynthetic thiolases, also known as acetoacetyl-CoA thiolases (AACT), catalyse a reversible Claisen-type condensation of two acetyl-CoA ...
Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein
Isopentenyl diphosphate (IPP, 9), the fundamental unit in terpenoid biosynthesis, was widely accepted to be solely formed through the mevalonate pathway.
Functional analysis of the Arabidopsis thaliana GCPE protein involved in plastid isoprenoid biosynthesis
Plastid isoprenoids are synthesized via the 2-C-methyl-D-erythritol 4-phosphate pathway. A few years after its discovery, most of the Escherichia coli genes involved in the pathway have been identified, including gcpE. In ...
Enhanced flux through the methylerythritol phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase
The methylerythritol 4-phosphate (MEP) pathway synthesizes the precursors for an astonishing diversity of plastid isoprenoids, including the major photosynthetic pigments chlorophylls and carotenoids. Since the identification ...
What are the current trends in G protein-coupled receptor targeted drug discovery?
G protein-coupled receptors (GPCRs) constitute a superfamily of membrane proteins which have in common the presence of 7 transmembrane α-helices linked by several intra- and extracellular loops. This particular structure ...
Mevalonate Biosynthesis in Plants
A structural model for plant 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) has been proposed on the basis of primary sequence comparisons. In order to define the topology of Arabidopsis HMGR in the estrogen receptor ...
Cloning and characterization of the gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose-5-phosphate, a common precursor for isoprenoid, thiamin and pyridoxol biosynthesis
For many years it was accepted that isopentenyl diphosphate, the common precursor of all isoprenoids, was synthesized through the well known acetate/mevalonate pathway. However, recent studies have shown that some bacteria, ...
Biosynthesis of isoprenoid precursors in arabidopsis
Isoprenoids are the most functionally and structurally diverse group of plant metabolites reported to date. Many of them participate in essential processes, like respiration (ubiquinone), photosynthesis (carotenoids, ...
Flexibility in PAM recognition expands DNA targeting in xCas9
xCas9 is an evolved variant of the CRISPR-Cas9 genome editing system, engineered to improve specificity and reduce undesired off-target effects. How xCas9 expands the DNA targeting capability of Cas9 by recognising a series ...
The pseudotorsional space of RNA
The characterization of the conformational landscape of the RNA backbone is rather complex due to the ability of RNA to assume a large variety of conformations. These backbone conformations can be depicted by pseudotorsional ...
The hpx genetic system for hypoxanthine assimilation as a nitrogen source in Klebsiella pneumoniae: gene organization and transcriptional regulation.
Growth experiments showed that adenine and hypoxanthine can be used as nitrogen sources by several strains of K. pneumoniae under aerobic conditions. The assimilation of all nitrogens from these purines indicates that the ...
A method for the measurement of lactate, glycerol and fatty acid production from 14C-glucose in primary cultures of rat epididymal adipocytes.
We have developed a method for the analysis of the main metabolic products of utilization of glucose by isolated adipocytes. They were incubated for 24 h with 14C-glucose. The final label distribution and cold levels of ...
