Biomedicina: Recent submissions
Now showing items 181-200 of 779
Ribonucleoprotein HNRNPA2B1 interacts with and regulates oncogenic KRAS in Pancreatic Ductal Adenocarcinoma Cells.
BACKGROUND & AIMS: Development of pancreatic ductal adenocarcinoma (PDAC) involves activation of c-Ki-ras2 Kirsten rat sarcoma oncogene homolog (KRAS) signaling, but little is known about the roles of proteins that regulate ...
Embryonic self-fracking
From a broken bone to a major earthquake, the fracture of a material usually means trouble. However, in some practical applications engineers have learned how to harness the mechanisms of fracture. This is illustrated by ...
Cardiac fibroblasts and mechanosensation in heart development, health and disease
The term 'mechanosensation' describes the capacity of cells to translate mechanical stimuli into the coordinated regulation of intracellular signals, cellular function, gene expression and epigenetic programming. This ...
The plasma membrane as a mechanochemical transducer
Cells are constantly submitted to external mechanical stresses, which they must withstand and respond to. By forming a physical boundary between cells and their environment that is also a biochemical platform, the plasma ...
Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility
Several factors present in the extracellular environment regulate epithelial cell adhesion and dynamics. Among them, growth factors such as EGF, upon binding to their receptors at the cell surface, get internalized and ...
Parametric modeling of mechanical effects on circadian oscillators
Circadian rhythms are archetypal examples of nonlinear oscillations. While these oscillations are usually attributed to circuits of biochemical interactions among clock genes and proteins, recent experimental studies reveal ...
Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity
Cell function depends on tissue rigidity, which cells probe by applying and transmitting forces to their extracellular matrix, and then transducing them into biochemical signals. Here we show that in response to matrix ...
Measuring mechanical stress in living tissues
Living tissues are active, multifunctional materials capable of generating, sensing, withstanding and responding to mechanical stress. These capabilities enable tissues to adopt complex shapes during development, to sustain ...
Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration
Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although ...
Hydraulic fracturing in cells and tissues: fracking meets cell biology
The animal body is largely made of water. A small fraction of body water is freely flowing in blood and lymph, but most of it is trapped in hydrogels such as the extracellular matrix (ECM), the cytoskeleton, and chromatin. ...
Durotaxis
In this Primer, Sunyer and Trepat introduce durotaxis, the mode of migration by which cells follow gradients of extracellular matrix stiffness.
Endocytic reawakening of motility in jammed epithelia.
Dynamics of epithelial monolayers has recently been interpreted in terms of a jamming or rigidity transition. How cells control such phase transitions is, however, unknown. Here we show that RAB5A, a key endocytic protein, ...
Mesoscale physical principles of collective cell organization
We review recent evidence showing that cell and tissue dynamics are governed by mesoscale physical principles. These principles can be understood in terms of simple state diagrams in which control variables include force, ...
TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4
Mechanically induced signaling pathways are important drivers of tumor progression. However, if and how mechanical signals affect metastasis or therapy response remains poorly understood. We previously found that the ...
Optogenetic generation of leader cells reveals a force-velocity relation for collective cell migration
During development, wound healing and cancer invasion, migrating cell clusters feature highly protrusive leader cells at their front. Leader cells are thought to pull and direct their cohort of followers, but whether their ...
Molecular clutch drives cell response to surface viscosity
Cell response to matrix rigidity has been explained by the mechanical properties of the actin-talin-integrin-fibronectin clutch. Here the molecular clutch model is extended to account for cell interactions with purely ...
Cell Migration Driven by Cooperative Substrate Deformation Patterns
Most eukaryotic cells sense and respond to the mechanical properties of their surroundings. This can strongly influence their collective behavior in embryonic development, tissue function, and wound healing. We use a ...
Collective cell durotaxis emerges from long-range intercellular force transmission
The ability of cells to follow gradients of extracellular matrix stiffness-durotaxis-has been implicated in development, fibrosis, and cancer. Here, we found multicellular clusters that exhibited durotaxis even if isolated ...
Technology for noninvasive mechanical ventilation: looking into the black box
Current devices for providing noninvasive respiratory support contain sensors and built-in intelligence for automatically modifying ventilation according to the patient's needs. These devices, including automatic continuous ...
Quantifying forces in cell biology
Cells exert, sense, and respond to physical forces through an astounding diversity of mechanisms. Here we review recently developed tools to quantify the forces generated by cells. We first review technologies based on ...
