Sistema de Salut de Catalunya
Institut Català de la Salut
[Colomé N, Martín L, Canals F] ProteoRed-ISCIII, Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Abian J] ProteoRed-ISCIII, Instituto de Investigaciones Biomédicas de Barcelona, IIBB-CSIC/IDIBAPS, Barcelona, Spain. [Aloria K] ProteoRed-ISCIII, Proteomics Core Facility-SGIKER, University of the Basque Country (UPV/EHU), Leioa, Spain. [Arizmendi JM] Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain. [Barceló-Batllori S] ProteoRed-ISCIII, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain. [Braga-Lagache S] Department for BioMedical Research (DBMR), Proteomics and Mass Spectrometry Core Facility, University of Bern, Bern, Switzerland
Vall d'Hebron Barcelona Hospital Campus
2023-06-07T10:07:33Z
2023-06-07T10:07:33Z
2022-01-16
Phosphoproteome
Fosfoproteoma
Fosfoproteoma
Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.
ProteoRed, PRB3 is supported by grant PT17/0019/0001, of the PE I+D+i 2013-2016, funded by ISCIII and ERDF.
Article
Published version
English
Proteòmica; Fosforilació; Epidemiologia; PHENOMENA AND PROCESSES::Chemical Phenomena::Biochemical Phenomena::Phosphorylation; DISCIPLINES AND OCCUPATIONS::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics; ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results; FENÓMENOS Y PROCESOS::fenómenos químicos::fenómenos bioquímicos::fosforilación; DISCIPLINAS Y OCUPACIONES::disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::proteómica; TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::métodos epidemiológicos::diseño de la investigación epidemiológica::reproducibilidad de los resultados
Elsevier
Journal of Proteomics;251
https://doi.org/10.1016/j.jprot.2021.104409
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
