dc.contributor
Institut Català de la Salut
dc.contributor
[Colomé N, Martín L, Canals F] ProteoRed-ISCIII, Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Abian J] ProteoRed-ISCIII, Instituto de Investigaciones Biomédicas de Barcelona, IIBB-CSIC/IDIBAPS, Barcelona, Spain. [Aloria K] ProteoRed-ISCIII, Proteomics Core Facility-SGIKER, University of the Basque Country (UPV/EHU), Leioa, Spain. [Arizmendi JM] Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain. [Barceló-Batllori S] ProteoRed-ISCIII, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain. [Braga-Lagache S] Department for BioMedical Research (DBMR), Proteomics and Mass Spectrometry Core Facility, University of Bern, Bern, Switzerland
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Colome Calls, Núria
dc.contributor.author
Abian, Joaquin
dc.contributor.author
Aloria, Kerman
dc.contributor.author
Arizmendi, Jesus M
dc.contributor.author
Barcelo-Batllori, Silvia
dc.contributor.author
Braga-Lagache, Sophie
dc.contributor.author
Martín Castel, Luna
dc.contributor.author
Canals Suris, Francesc
dc.date.accessioned
2025-10-25T05:39:45Z
dc.date.available
2025-10-25T05:39:45Z
dc.date.issued
2023-06-07T10:07:33Z
dc.date.issued
2023-06-07T10:07:33Z
dc.date.issued
2022-01-16
dc.identifier
Colomé N, Abian J, Aloria K, Arizmendi JM, Barceló-Batllori S, Braga-Lagache S, et al. Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis. J Proteomics. 2022 Jan 16;251:104409.
dc.identifier
https://hdl.handle.net/11351/9677
dc.identifier
10.1016/j.jprot.2021.104409
dc.identifier
000721676300010
dc.identifier.uri
http://hdl.handle.net/11351/9677
dc.description.abstract
Phosphoproteome
dc.description.abstract
Fosfoproteoma
dc.description.abstract
Fosfoproteoma
dc.description.abstract
Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.
dc.description.abstract
ProteoRed, PRB3 is supported by grant PT17/0019/0001, of the PE I+D+i 2013-2016, funded by ISCIII and ERDF.
dc.format
application/pdf
dc.relation
Journal of Proteomics;251
dc.relation
https://doi.org/10.1016/j.jprot.2021.104409
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
PHENOMENA AND PROCESSES::Chemical Phenomena::Biochemical Phenomena::Phosphorylation
dc.subject
DISCIPLINES AND OCCUPATIONS::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results
dc.subject
FENÓMENOS Y PROCESOS::fenómenos químicos::fenómenos bioquímicos::fosforilación
dc.subject
DISCIPLINAS Y OCUPACIONES::disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::proteómica
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::métodos epidemiológicos::diseño de la investigación epidemiológica::reproducibilidad de los resultados
dc.title
Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
