Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/139039
dc.contributor.author | López Aladid, Rubén |
---|---|
dc.contributor.author | Guiu, Alba |
dc.contributor.author | Mosquera, Maria Mar |
dc.contributor.author | López Medrano, Francisco |
dc.contributor.author | Cofán Pujol, Federico |
dc.contributor.author | Linares, Laura |
dc.contributor.author | Torre Cisneros, Julián |
dc.contributor.author | Vidal, Elisa |
dc.contributor.author | Moreno Camacho, Ma. Asunción |
dc.contributor.author | Aguado, José María |
dc.contributor.author | Cordero, Elisa |
dc.contributor.author | Martin Gandul, Cecilia |
dc.contributor.author | Carratalà, Jordi |
dc.contributor.author | Sabé, Nuria |
dc.contributor.author | Niubó, Jordi |
dc.contributor.author | Cervera, Carlos |
dc.contributor.author | Capón, Alicia |
dc.contributor.author | Cervilla, Anna |
dc.contributor.author | Santos, Marta |
dc.contributor.author | Bodro, Marta |
dc.contributor.author | Muñoz, Patricia |
dc.contributor.author | Farinas, Maria Carmen |
dc.contributor.author | Antón, Andrés |
dc.contributor.author | Aranzamendi, Maitane |
dc.contributor.author | Montejo, Miguel |
dc.contributor.author | Pérez Romero, Pilar |
dc.contributor.author | Len, Óscar |
dc.contributor.author | Marcos, Ma. Angeles |
dc.date | 2019-09-02T10:55:46Z |
dc.date | 2019-09-02T10:55:46Z |
dc.date | 2019-07-18 |
dc.date | 2019-08-02T18:00:48Z |
dc.identifier | 1932-6203 |
dc.identifier | 31318908 |
dc.identifier.uri | http://hdl.handle.net/2445/139039 |
dc.description | Objectives: The aim of this study was to identify CMV drug resistance mutations (DRM) in solid organ transplant (SOT) recipients with suspected resistance comparing next-generation sequencing (NGS) with Sanger sequencing and assessing risk factors and the clinical impact of resistance. Methods: Using Sanger sequencing as the reference method, we prospectively assessed the ability of NGS to detect CMV DRM in the UL97 and UL54 genes in a nationwide observational study from September 2013 to August 2016. Results: Among 44 patients recruited, 14 DRM were detected by Sanger in 12 patients (27%) and 20 DRM were detected by NGS, in 16 (36%). NGS confirmed all the DRM detected by Sanger. The additional six mutations detected by NGS were present in <20% of the sequenced population, being located in the UL97 gene and conferring high-level resistance to ganciclovir. The presence of DRM by NGS was associated with lung transplantation (p = 0.050), the administration of prophylaxis (p = 0.039), a higher mean time between transplantation and suspicion of resistance (p = 0.038) and longer antiviral treatment duration before suspicion (p = 0.024). However, the latter was the only factor independently associated with the presence of DRM by NGS in the multivariate analysis (OR 2.24, 95% CI 1.03 to 4.87). Conclusions: NGS showed a higher yield than Sanger sequencing for detecting CMV resistance mutations in SOT recipients. The presence of DRM detected by NGS was independently associated with longer antiviral treatment. |
dc.format | 13 p. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | Public Library of Science (PLoS) |
dc.relation | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0219701 |
dc.relation | PLoS ONE, 2019, vol. 14 , num. 7, p. e0219701 |
dc.relation | http://dx.doi.org/10.1371/journal.pone.0219701 |
dc.rights | cc by (c) López Aladid et al., 2019 |
dc.rights | http://creativecommons.org/licenses/by/3.0/es/ |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Citomegalovirus |
dc.subject | Trasplantament hepàtic |
dc.subject | Cytomegaloviruses |
dc.subject | Hepatic transplantation |
dc.title | Improvement in detecting cytomegalovirus drug resistance mutations in solid organ transplant recipients with suspected resistance using next generation sequencing |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |