Differential microRNA expression profile between stimulated PBMCs from HIV-1 infected elite controllers and viremic progressors

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RECERCAT Home > Universitat de Barcelona > IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer > Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) > View document

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dc.contributor.author	Egaña Gorroño, Lander
dc.contributor.author	Escribà, Tuixent
dc.contributor.author	Boulanger, Nicolas
dc.contributor.author	Crespo Guardo, Alberto
dc.contributor.author	León García, Agathe
dc.contributor.author	Bargalló, Manel Enric
dc.contributor.author	García, Felipe
dc.contributor.author	Gatell, José M.
dc.contributor.author	Plana Prades, Montserrat
dc.contributor.author	Arnedo, Mireia
dc.date	2018-03-15T19:15:02Z
dc.date	2018-03-15T19:15:02Z
dc.date	2014
dc.date	2018-03-15T19:15:02Z
dc.identifier	1932-6203
dc.identifier	649154
dc.identifier	25225963
dc.identifier.uri	http://hdl.handle.net/2445/120810
dc.description	BACKGROUND: The emerging relationship between microRNAs (miRNA) and viral-control is a topic of interest in the field of HIV. Host-genome might play an important role in the control of viremia. The aim of this study was to assess the specific miRNA profile that could contribute to the control of HIV replication in Elite Controllers. RESULTS: After adequate normalization, expression profile of 286 human miRNAs (hsa-miR) was evaluated in phytohaemagglutinin-stimulated PBMCs from 29 individuals classified in 4 groups: 8 elite controllers (EC; viral load <50 cp/ml without treatment), 8 viremic progressors (VP; VL>5000 cp/ml without treatment), 8 patients under antiretroviral treatment (ART; VL<200 cp/ml) and 5 uninfected individuals (HIV-) through TaqMan Array Human microRNA Cards v3.0. A differential expression pattern consisting of 23 miRNAs became significantly different when comparing EC and VP. Profiling analysis segregated the population in two different blocks: while EC and HIV- clustered together in the same block (EC/HIV-_block 1), VP and ART individuals clustered together in a second block (VP/ART_block 2). Two inversely expressed miRNA patterns were determined within those two blocks: a set of 4 miRNAs (hsa-miR-221, -27a, -27b and -29b) was up-expressed in EC/HIV-_block and down-expressed in VP/ART_block while 19 miRNAs were down-expressed in block 1 and up-expressed in block 2. Differential miRNAs were successfully validated through individual RT-qPCR assays. CONCLUSIONS: Profile in EC resembled HIV- and differentially clusters with VP and ART. Therefore, differential clustering does not rely on undetectable viremia.
dc.format	10 p.
dc.format	application/pdf
dc.language	eng
dc.publisher	Public Library of Science (PLoS)
dc.relation	Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0106360
dc.relation	PLoS One, 2014, vol. 9, num. 9, p. e106360
dc.relation	https://doi.org/10.1371/journal.pone.0106360
dc.rights	cc-by (c) Egaña Gorroño, Lander et al., 2014
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Expressió gènica
dc.subject	Micro RNAs
dc.subject	Infeccions per VIH
dc.subject	VIH (Virus)
dc.subject	Gene expression
dc.subject	MicroRNAs
dc.subject	HIV infections
dc.subject	HIV (Viruses)
dc.title	Differential microRNA expression profile between stimulated PBMCs from HIV-1 infected elite controllers and viremic progressors
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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