Título:
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Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1
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Autor/a:
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Perez-Zsolt, Daniel; Cantero, Jon; Erkizia, Itziar; Benet, Susana; Pino, M.; Serra-Peinado, Carla; Hernández Gallego, Alba; Castellvi, Josep; Tapia, G.; Arnau-Saz, V.; Garrido, J.; Tarrats, Antoni; Buzón, Maria José; Martinez-Picado, J.; Izquierdo Useros, Nuria; Genescà Ferrer, Meritxell.
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Abstract:
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Altres ajuts: JM-P and NI-U are supported by the Spanish Secretariat of State of Research, Development and Innovation through grant SAF2016-80033-R. MG is supported by a Marie Curie Career Integration Grant (CIG) from the European Commission and by the Pla estratègic de recerca i innovació en salut (PERIS), from the Catalan government. |
Abstract:
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Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies. |
Materia(s):
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-Cervix -Siglec-1 -HIV-1 -Trans-infection -Myeloid cells |
Derechos:
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open access
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Article |
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Uri:
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https://ddd.uab.cat/record/223637
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