dc.contributor.author |
Dey, Priyanka |
dc.contributor.author |
Fabri Faja, Nuria |
dc.contributor.author |
Calvo Lozano, Olalla |
dc.contributor.author |
Terborg, Roland A. |
dc.contributor.author |
Belushkin, Alexander |
dc.contributor.author |
Yesilköy, Filiz |
dc.contributor.author |
Fàbrega-Santamaria, Anna |
dc.contributor.author |
Ruiz Rodríguez, Juan Carlos |
dc.contributor.author |
Ferrer Roca, Ricard |
dc.contributor.author |
González López, Juan José |
dc.contributor.author |
Estévez, M. Carmen |
dc.contributor.author |
Altug, Hatice |
dc.contributor.author |
Pruneri, Valerio |
dc.contributor.author |
Lechuga, Laura |
dc.date |
2019 |
dc.identifier |
https://ddd.uab.cat/record/218093 |
dc.identifier |
10.1021/acssensors.8b00789 |
dc.identifier |
oai:ddd.uab.cat:218093 |
dc.identifier |
85059409753 |
dc.identifier |
2379-3694v4n1p52 |
dc.identifier |
000457203200007 |
dc.identifier |
52567905 |
dc.identifier |
oai:egreta.uab.cat:publications/5bf8532f-0732-4ed4-bba9-7f15b3b9f110 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
European Commission 644956 |
dc.relation |
info:eu-repo/grantAgreement/MINECO/SEV-2013-0295 |
dc.relation |
info:eu-repo/grantAgreement/MINECO/FICTS-1420-27 |
dc.relation |
info:eu-repo/grantAgreement/MINECO/SEV-2015-0522 |
dc.relation |
info:eu-repo/grantAgreement/MINECO/TEC2016-75080-R |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1634 |
dc.relation |
ACS Sensors ; Vol. 4, Issue 1 (January 2019), p. 52-60 |
dc.rights |
open access |
dc.rights |
Tots els drets reservats. |
dc.rights |
https://rightsstatements.org/vocab/InC/1.0/ |
dc.subject |
Bacteria |
dc.subject |
Sepsis |
dc.subject |
Microarray |
dc.subject |
Label-free detection |
dc.subject |
Nanoplasmonics |
dc.subject |
Plasma samples |
dc.title |
Label-free Bacteria Quantification in Blood Plasma by a Bioprinted Microarray Based Interferometric Point-of-Care Device |
dc.type |
Article |
dc.description.abstract |
Existing clinical methods for bacteria detection lack speed, sensitivity, and, importantly, point-of-care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Here we report a portable PoC device based on lens-free interferometric microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample-to-data turnaround time of 40 min. Our technology features detection sensitivity of a single bacterial cell both in buffer and in diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and noninfectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality. |