dc.contributor.author |
Campo, Sonia |
dc.contributor.author |
Peris Peris, Cristina |
dc.contributor.author |
Montesinos Barreda, Laura |
dc.contributor.author |
Peñas Civit, Gisela |
dc.contributor.author |
Messeguer, Joaquima |
dc.contributor.author |
San Segundo, Blanca |
dc.date |
2011 |
dc.identifier |
https://ddd.uab.cat/record/184764 |
dc.identifier |
urn:10.1093/jxb/err328 |
dc.identifier |
urn:oai:ddd.uab.cat:184764 |
dc.identifier |
urn:pmid:22016430 |
dc.identifier |
urn:pmcid:PMC3254693 |
dc.identifier |
urn:pmc-uid:3254693 |
dc.identifier |
urn:articleid:14602431v63p983 |
dc.identifier |
urn:altmetric_id:862110 |
dc.identifier |
urn:scopus_id:84855876430 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:3254693 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Ministerio de Ciencia e Innovación BIO2009-08719 |
dc.relation |
Ministerio de Ciencia e Innovación CSD2007-00036 |
dc.relation |
Ministerio de Ciencia e Innovación BIO2006-05583 |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-09626 |
dc.relation |
Journal of experimental botany ; Vol. 63, issue 2 (Jan. 2012), p. 983-999 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by-nc/4.0/ |
dc.subject |
14-3-3 proteins |
dc.subject |
Abiotic stress |
dc.subject |
Biotic stress |
dc.subject |
Early endosomes |
dc.subject |
Fusarium verticillioides |
dc.subject |
Drought |
dc.subject |
Magnaporthe oryzae |
dc.subject |
Oryza sativa |
dc.subject |
Zea mays |
dc.subject |
ZmPR4 promoter |
dc.title |
Expression of the maize ZmGF14-6 gene in rice confers tolerance to drought stress while enhancing susceptibility to pathogen infection |
dc.type |
Article |
dc.description.abstract |
14-3-3 proteins are found in all eukaryotes where they act as regulators of diverse signalling pathways associated with a wide range of biological processes. In this study the functional characterization of the ZmGF14-6 gene encoding a maize 14-3-3 protein is reported. Gene expression analyses indicated that ZmGF14-6 is up-regulated by fungal infection and salt treatment in maize plants, whereas its expression is down-regulated by drought stress. It is reported that rice plants constitutively expressing ZmGF14-6 displayed enhanced tolerance to drought stress which was accompanied by a stronger induction of drought-associated rice genes. However, rice plants expressing ZmGF14-6 either in a constitutive or under a pathogen-inducible regime showed a higher susceptibility to infection by the fungal pathogens Fusarium verticillioides and Magnaporthe oryzae. Under infection conditions, a lower intensity in the expression of defence-related genes occurred in ZmGF14-6 rice plants. These findings support that ZmGF14-6 positively regulates drought tolerance in transgenic rice while negatively modulating the plant defence response to pathogen infection. Transient expression assays of fluorescently labelled ZmGF14-6 protein in onion epidermal cells revealed a widespread distribution of ZmGF14-6 in the cytoplasm and nucleus. Additionally, colocalization experiments of fluorescently labelled ZmGF14-6 with organelle markers, in combination with cell labelling with the endocytic tracer FM4-64, revealed a subcellular localization of ZmGF14-6 in the early endosomes. Taken together, these results improve our understanding of the role of ZmGF14-6 in stress signalling pathways, while indicating that ZmGF14-6 inversely regulates the plant response to biotic and abiotic stresses. |