Title:
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Production of cecropin A antimicrobial peptide in rice seed endosperm
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Author:
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Bundó Barberà, Mireia; Montesinos Barreda, Laura; Izquierdo, Esther; Campo, Sonia; Mieulet, Delphine; Guiderdoni, Emmanuel; Rossignol, Michel; Badosa Romañó, Esther; Montesinos Seguí, Emilio; San Segundo, Blanca; Coca López, María
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Abstract:
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Background: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. - Results: transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. - Conclusions: rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation. |
Subject(s):
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-Rice -Antimicrobial peptides -Cecropin A -Endosperm -Protein bodies -Pathogen resistance -Fusarium verticillioides -Dickeya dadantii -Oryza sativa |
Rights:
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open access
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Document type:
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Article |
Published by:
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Uri:
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https://ddd.uab.cat/record/175933
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