dc.contributor.author |
Mongui, Álvaro |
dc.contributor.author |
Pérez Llanos, Francy J. |
dc.contributor.author |
Yamamoto, Marcio M. |
dc.contributor.author |
Lozano, Marcela |
dc.contributor.author |
Zambrano, Maria M. |
dc.contributor.author |
Portillo, Patricia del |
dc.contributor.author |
Fernández Becerra, Carmen |
dc.contributor.author |
Restrepo, Silvia |
dc.contributor.author |
Portillo Obando, Hernando A. del |
dc.contributor.author |
Junca, Howard |
dc.date |
2015 |
dc.identifier |
https://ddd.uab.cat/record/163154 |
dc.identifier |
urn:10.1186/s12936-015-0748-6 |
dc.identifier |
urn:oai:ddd.uab.cat:163154 |
dc.identifier |
urn:pmid:26040274 |
dc.identifier |
urn:scopus_id:84934916416 |
dc.identifier |
urn:wos_id:000355899300001 |
dc.identifier |
urn:altmetric_id:4097437 |
dc.identifier |
urn:pmc-uid:4464701 |
dc.identifier |
urn:pmcid:PMC4464701 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:4464701 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
European Commission KBBE/2009 |
dc.relation |
Malaria journal ; Vol. 14 Núm. 233 (June 2015) |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/3.0/ |
dc.subject |
Malaria |
dc.subject |
Metagenomics |
dc.subject |
Functional screening |
dc.subject |
Anti-malarial drugs |
dc.subject |
Synthetic gene design |
dc.subject |
Dermaseptin 4 |
dc.subject |
Bioluminescent Plasmodium falciparum |
dc.title |
Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries |
dc.type |
Article |
dc.description.abstract |
Ajuts: Departamento Administrativo de Ciencias, Tecnología e Innovación (Colciencias), República de Colombia; Convocatoria 489 - 2009, Código 657048925406, Contrato de financiación RC. 427 - 2009 Colciencias - CorpoGen; Programa de Asistencias Graduadas de Universidad de los Andes, Bogotá, Colombia; i Programa Jóvenes Investigadores de Colciencias |
dc.description.abstract |
BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed |