Title:
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Development of a genetic tool for functional screening of
anti-malarial bioactive extracts in metagenomic libraries
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Author:
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Mongui, Alvaro; Pérez-Llanos, Francy J.; Yamamoto, Marcio M.; Lozano, Marcela; Zambrano, Maria M.; Portillo, Patricis Del; Fernández Becerra, Carmen; Restrepo, Silvia; Junca, Howard
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Other authors:
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Del Portillo, Hernando A. |
Abstract:
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BACKGROUND: The chemical treatment of Plasmodium falciparum for
human infections is losing efficacy each year due to the rise of
resistance. One possible strategy to find novel anti-malarial
drugs is to access the largest reservoir of genomic biodiversity
source on earth present in metagenomes of environmental
microbial communities. METHODS: A bioluminescent P. falciparum
parasite was used to quickly detect shifts in viability of
microcultures grown in 96-well plates. A synthetic gene encoding
the Dermaseptin 4 peptide was designed and cloned under tight
transcriptional control in a large metagenomic insert context
(30 kb) to serve as proof-of-principle for the screening
platform. RESULTS: Decrease in parasite viability consistently
correlated with bioluminescence emitted from parasite
microcultures, after their exposure to bacterial extracts
containing a plasmid or fosmid engineered to encode the
Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new
technical platform to access the anti-malarial potential in
microbial environmental metagenomes has been developed. |
Subject(s):
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-Genòmica -Genètica microbiana -Malària -Plasmodium falciparum -Resistència als medicaments -Efecte dels medicaments sobre els microorganismes -Genomics -Microbial genetics -Malaria -Plasmodium falciparum -Drug resistance -Effect of drugs on microorganisms |
Rights:
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cc by (c) Mongui et al., 2015
http://creativecommons.org/licenses/by/3.0/es/ |
Document type:
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Article Article - Published version |
Published by:
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BioMed Central
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