dc.contributor.author |
Martínez, Ignacio |
dc.contributor.author |
Campoy Sánchez, Susana |
dc.contributor.author |
Tort, Meritxell |
dc.contributor.author |
Llagostera Casas, Montserrat |
dc.contributor.author |
Petrov, Dmitri |
dc.date |
2013 |
dc.identifier |
https://ddd.uab.cat/record/142361 |
dc.identifier |
urn:10.1371/journal.pone.0061630 |
dc.identifier |
urn:oai:ddd.uab.cat:142361 |
dc.identifier |
urn:pmid:23637869 |
dc.identifier |
urn:recercauab:ARE-75743 |
dc.identifier |
urn:articleid:19326203v8n4e61630 |
dc.identifier |
urn:scopus_id:84876941629 |
dc.identifier |
urn:wos_id:000321662800010 |
dc.identifier |
urn:altmetric_id:1422239 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/d314ecdd-249b-4a23-b8c2-18af4d02af74 |
dc.identifier |
urn:pmc-uid:3639288 |
dc.identifier |
urn:pmcid:PMC3639288 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:3639288 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Ministerio de Economía y Competitividad FIS2008-00114 |
dc.relation |
Ministerio de Economía y Competitividad FIS2011-24409 |
dc.relation |
Ministerio de Economía y Competitividad BFU2011-23478 |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-1106 |
dc.relation |
PloS one ; Vol. 8 Issue 4 (April 2013), p. e61630 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/3.0/ |
dc.subject |
Flagellar rotation |
dc.subject |
Flagella |
dc.subject |
Bacterial genetics |
dc.subject |
Swimming |
dc.subject |
Pathogen motility |
dc.subject |
Chemotaxis |
dc.subject |
Oxygen |
dc.subject |
Cell motility |
dc.title |
A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern |
dc.type |
Article |
dc.description.abstract |
Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the Typhimurium mutant were characterized. |