Title:
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A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern
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Author:
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Martínez, Ignacio; Campoy Sánchez, Susana; Tort, Meritxell; Llagostera Casas, Montserrat; Petrov, Dmitri
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Abstract:
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Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the Typhimurium mutant were characterized. |
Subject(s):
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-Flagellar rotation -Flagella -Bacterial genetics -Swimming -Pathogen motility -Chemotaxis -Oxygen -Cell motility |
Rights:
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open access
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
https://creativecommons.org/licenses/by/3.0/ |
Document type:
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Article |
Published by:
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Share:
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Uri:
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https://ddd.uab.cat/record/142361
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