dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Tejero Villalba, Rut |
dc.contributor.author |
Navarro Ponz, Alfons |
dc.contributor.author |
Campayo Guillaumes, Marc |
dc.contributor.author |
Viñolas Segarra, Núria |
dc.contributor.author |
Marrades Sicart, Ramon Ma. |
dc.contributor.author |
Cordeiro, Anna |
dc.contributor.author |
Ruíz Martínez, Marc |
dc.contributor.author |
Santasusagna, Sandra |
dc.contributor.author |
Molins López-Rodó, Laureano |
dc.contributor.author |
Ramírez Ruz, J. (José) |
dc.contributor.author |
Monzó Planella, Mariano |
dc.date |
2020-01-23T12:30:56Z |
dc.date |
2020-01-23T12:30:56Z |
dc.date |
2014-07-08 |
dc.date |
2020-01-23T12:30:57Z |
dc.identifier.citation |
1932-6203 |
dc.identifier.citation |
642876 |
dc.identifier.uri |
http://hdl.handle.net/2445/148554 |
dc.format |
9 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Public Library of Science (PLoS) |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0101899 |
dc.relation |
PLoS One, 2014, vol. 9, num. 7, p. e101899 |
dc.relation |
https://doi.org/10.1371/journal.pone.0101899 |
dc.rights |
cc-by (c) Tejero Villalba, Rut et al., 2014 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es |
dc.subject |
Migració cel·lular |
dc.subject |
Pronòstic mèdic |
dc.subject |
Histologia |
dc.subject |
Cell migration |
dc.subject |
Prognosis |
dc.subject |
Histology |
dc.title |
miR-141 and miR-200c as markers of overall survival in early stage non-small cell lung cancer adenocarcinoma |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Several treatments in non-small cell lung cancer (NSCLC) are histology-dependent, and the need for histology-related markers is increasing. MicroRNAs (miRNAs) are promising molecular markers in multiple cancers and show differences in expression depending on histological subtype. The miRNA family miR-200 has been associated with the regulation of epithelial-mesenchymal (EMT)/mesenchymal-epithelial transition (MET). EMT involves profound phenotypic changes that include the loss of cell-cell adhesion, the loss of cell polarity, and the acquisition of migratory and invasive properties that facilitates metastasis. A dual role for the miR-200 family in the prognosis of several tumors has been related to tumor cell origin. However, the prognostic role and function of miR-200 family in early-stage NSCLC adenocarcinoma and squamous cell carcinoma (SCC) have not been well established. Methods: miRNA expression was determined using TaqMan assays in 155 tumors from resected NSCLC patients. Functional studies were conducted in three NSCLC cell lines: H23, A-549 and HCC-44. Results: High miR-200c expression was associated with shorter overall survival (OS) in the entire cohort (p = 0.024). High miR-200c (p = 0.0004) and miR-141 (p = 0.009) expression correlated with shorter OS in adenocarcinoma - but not in SCC. In the multivariate analysis, a risk score based on miR-141 and miR-200c expression emerged as an independent prognostic factor for OS in the entire cohort (OR, 2.787; p = 0.033) and in adenocarcinoma patients (OR, 10.649; p = 0.002). Functional analyses showed that miR-200c, was related to mesenchymal-epithelial transition (MET) and affected cell migration and E-cadherin levels, while overexpression of miR-141 reduced KLF6 protein levels and produced an increase of secretion of VEGFA in vitro (H23, p = 0.04; A-549, p = 0.03; HCC-44, p = 0.02) and was associated with higher blood microvessel density in patient tumor samples (p<0.001). Conclusion: High miR-141 and miR-200c expression are associated with shorter OS in NSCLC patients with adenocarcinoma through MET and angiogenesis. |