dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
López Cristoffanini, Camilo Alonso |
dc.contributor.author |
Serrat Gurrera, Xavier Victor |
dc.contributor.author |
Jáuregui, Olga |
dc.contributor.author |
Nogués Mestres, Salvador |
dc.contributor.author |
López Carbonell, Marta |
dc.date |
2019-09-18T13:59:20Z |
dc.date |
2019-09-18T13:59:20Z |
dc.date |
2019-06-07 |
dc.date |
2019-09-18T13:59:20Z |
dc.identifier.citation |
1664-462X |
dc.identifier.citation |
683732 |
dc.identifier.uri |
http://hdl.handle.net/2445/140443 |
dc.format |
14 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Frontiers Media |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.3389/fpls.2019.00733 |
dc.relation |
Frontiers in Plant Science, 2019, vol. 10, p. 733-000 |
dc.relation |
https://doi.org/10.3389/fpls.2019.00733 |
dc.rights |
cc-by (c) López Cristoffanini, Camilo Alonso et al., 2019 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es |
dc.subject |
Arròs |
dc.subject |
Biotecnologia alimentària |
dc.subject |
Rice |
dc.subject |
Food biotechnology |
dc.title |
Phytohormone Profiling method for rice: effects of GA20ox mutation on the gibberellin content of japonica rice varieties |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Gibberellins (GAs) are a very important group of phytohormones involved in seed germination, vegetative growth, flowering, and fruit development, being only 4 of the 136 known bioactives: GA1, GA3, GA4 and GA7. It has been evidenced that mutations in the OsGA20ox-2 gene produce rice (Oryza sativa) dwarf varieties, which were one of the main pillars of the green revolution. In this work two main objectives were proposed: (i) develop a rapid and broad phytohormone profiling method and (ii) to study the effects on the GA content of the GA20ox-2 mutation in several rice developmental stages using three varieties (tall variety, elite variety, mutated variety). A phytohormone extraction using an SPE step and HPLC-MS/MS detection using a QqQ instrument was determined which resulted in limits of detection (LOD) and limits of quantification (LOQ) for GAs that varied between 0.1-0.7 and 0.3-2.3 pg/g of rice sample respectively, allowing highly sensitive phytohormones detection in samples. Moreover, a good reproducibility was obtained for the GAs as relative standard deviations (RSD) for a 40 ng/mL pattern varied between 0.3-0.9%. Notoriously, GA1 was absent in the coleoptile and GA4 was the GA with higher content in the majority of developmental stages. We also observed a huge large content increment increase of the 4 bioactive GAs in the internode of the flag leaf of the mutated variety that allowinged it to attain reach the same height as the elite variety. Therefore, we provide a rapid and broad phytohormonal profiling method and evidence that the GA20ox-2 mutation is not the only factor generating dwarf varieties. To our knowledge, this is the first study that it has been reported such a high number of analyzed gibberellins at the same time in rice (Oryza sativa L. ssp. japonica)Oryza sativa japonica). |