dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Badiola Mateos, Maider |
dc.contributor.author |
Hervera Abad, Arnau |
dc.contributor.author |
Río Fernández, José Antonio del |
dc.contributor.author |
Samitier i Martí, Josep |
dc.date |
2019-08-26T11:01:11Z |
dc.date |
2019-08-26T11:01:11Z |
dc.date |
2018-12-11 |
dc.date |
2019-08-26T11:01:11Z |
dc.identifier.citation |
2296-4185 |
dc.identifier.citation |
685893 |
dc.identifier.uri |
http://hdl.handle.net/2445/138717 |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Frontiers Media |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.3389/fbioe.2018.00194 |
dc.relation |
Frontiers in Bioengineering and Biotechnology, 2018, vol. 6, num. 194 |
dc.relation |
https://doi.org/10.3389/fbioe.2018.00194 |
dc.rights |
cc-by (c) Badiola Mateos, Maider et al., 2018 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es |
dc.subject |
Malalties neuromusculars |
dc.subject |
Aparell locomotor |
dc.subject |
Bioenginyeria |
dc.subject |
Neuromuscular diseases |
dc.subject |
Musculoskeletal system |
dc.subject |
Bioengineering |
dc.title |
Challenges and future prospects on 3D in-vitro modeling of the neuromuscular circuit |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Movement of skeletal-muscle fibers is generated by the coordinated action of several cells taking part within the locomotion circuit (motoneurons, sensory-neurons, Schwann cells, astrocytes, microglia, and muscle-cells). Failure s in any part of this circuit could impede or hinder coordinated muscle movement and cause a neu romuscular disease (NMD) or determine its severity. Studying fragments of the circuit cannot provide a comprehensive and complete view of the pathological process. We trace the historic developments of studies focused on in-vitro modeling of the spinal-locomotion circuit and how bioengineered innovative technologies show advantages for an accurate mimicking of hysiological conditions of spinal-locomotion circuit. New developments on compartmentalized microfluidic culture systems (cμFCS), the use of human induced pluripotent stem cells (hiPSCs) and 3D cell-cultures are analyzed. We finally address limitations of current study models and three main challenges on neuromuscular studies: (i) mimic the whole spinal-locomotion circuit including all cell-types involved and the evaluation of independent and interdependent roles of each one; (ii) mimic the neurodegenerative response of mature neurons in-vitro as it occurs in-vivo ; and (iii) develop, tune, implement, and combine cμFCS, hiPSC, and 3D-culture technologies to ultimately create patient-specific complete, translational, and reliable NMD in-vitro model. Overcoming these challenges would significantly facilitate understanding the events taking place in NMDs and accelerate the process of finding new therapies. |