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Title:	Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine
Author:	Barbosa Torralbo, José; Sanz Nebot, María Victoria; Benavente Moreno, Fernando J. (Julián); Giménez, Estela
Other authors:	Universitat de Barcelona
Abstract:	Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms.
Subject(s):	-Electroforesi capil·lar -Glicoproteïnes -Espectrometria de masses -Proteïnes -Capillary electrophoresis -Glycoproteins -Mass spectrometry -Proteins
Rights:	, 2006
Document type:	Article
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