Accommodative response and its possible role in myopia development has been explored through the study of the microfluctuations of accommodation, which are commonly divided in high (1.0 to 2.3 Hz) and low (0.1 to 0.6 Hz) frequency components. Previous research efforts have evidenced that a certain percentage of the amplitude of microfluctuations seems not to originate in the accommodative response. We aimed to develop and test a new approach to reduce this non-accommodative noise. For this purpose, ten healthy participants were enrolled to determine the difference between the amplitude of microfluctuations at near and distance for each range of frequencies, which was defined as the relative amplitude of microfluctuations. The findings support the exploration of the relative rather than absolute values of the amplitude of microfluctuations to better understand the contribution of both accommodative and non-accommodative factors to microfluctuations.