Title:
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Development of a novel microsensor for the study of oxygen profiles in biofilms
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Author:
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Guimerà Villalba, Xavier; Moya Lara, Ana; Prats Alfonso, Elisabet; Dorado Castaño, Antonio David; Villa, Rosa; Gamisans Noguera, Javier; Gabriel Buguña, David; Gabriel Buguña, Gemma
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Other authors:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Minera, Industrial i TIC; Universitat Politècnica de Catalunya. BIOGAP - Grup de Tractament Biològic de Contaminants Gasosos i Olors |
Abstract:
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Understanding of the processes taking place inside biofilms is a key parameter to progress in the optimization of biofiltration technologies. This study was conducted with the aim of developing a novel dissolved oxygen (DO) microsensor specially designed for biofilms monitoring. The microsensor was fabricated through standard photolithography techniques, resulting in a microelectrodes array (MEA) of 11 gold circular working electrodes, with a diameter of 50 µm , and a gold reference electrode, which allows obtaining a snapshot oxygen profile of 1 mm of depth. The performance of the sensor was fully characterized under different conditions, inwhich the sensor presented high sensitivity and repeatability, and low detection and quantification limits. Monitoring of sensor performance showed a stable and reliable response. The developed sensor was used in obtaining micropofiles in an aerobic heterotrophic biofilm, showing similar response to Clark-type commercial microsensors. These studies concluded that the novel MEA sensor for DO monitoring allows obtaining oxygen profiles within biofilms, becoming a useful tool for the research of many biological applications. |
Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria química::Biotecnologia -Biofilms -Microelectrodes -Water -- Dissolved oxygen -Potentiometry -Microelectrodes array (MEA) -Dissolved oxygen -Biofilm profiling -Potentiometry -Biofilms -Microelèctrodes -Aigua -- Oxigen dissolt -Potenciometria |
Rights:
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Document type:
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Article - Submitted version Conference Object |
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