In this work, a strategy based on photo-sequencing batch operationwas used to select cyanobacteria over unsettled green algae in awastewater treatment system, evaluating for the first time the effect of hydraulic regimes on nutritional dynamics and microorganisms' competition. During 30 days of operation, an initial microalgae mixed consortia dominated by the green microalgae Scenedesmus sp. was cultivated in two different photo-sequencing batch reactors operated at hydraulic retention time (HRT) of 6 days (PSBR6) and 4 days (PSBR4) at a theoretical solids retention time (SRT) of 10 d. Both reactorswere compared with a semi-continuous reactor (SC10) operated at 10 d of HRT and 10 days of SRT (used as a control). The results indicated that PSBR6 and PSBR4 decreased Scenedesmus sp. population by 88% and 48%, respectively. However, only PSBR6 provided suitable conditions to select cyanobacteria from an initial green algae dominated culture. These conditions included volumetric loads of 11.72 mg TN L-1 d-1, 2.04mg TP L-1 d-1 and 53.31 mg TOC L-1 d-1. The remaining nutrients in the culture led also to a phosphorus limiting N:P ratio (34:1) that improved the increase of cyanobacteria from an initial 2% until 70% of the total population. In addition, PSBR6 reached a biomass production of 0.12 g L-1 d-1,while removing TN, TP and TOC by 58%, 83% and 85%, respectively. Conversely, the application of higher nutrients loads caused by lowerHRT (PSBR4) led to an increase of only 13% of cyanobacteriawhile SC10 remainedwith the same biomass composition during all the experimental time. Thus, this study showed that the dominance of cyanobacteria in microalgal-based wastewater treatment systems can be controlled by the operational and nutritional conditions

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