To access the full text documents, please follow this link: http://hdl.handle.net/2445/127751

Title:	Mitochondrial fragmentation in excitotoxicity requires ROCK activation
Author:	Martorell-Riera, A.; Segarra-Mondejar, M., Reina, M.; Reina del Pozo, Manuel; Martínez Estrada, Ofelia María; Soriano Zaragoza, Francesc X. (Francesc Xavier)
Other authors:	Universitat de Barcelona
Abstract:	Mitochondria morphology constantly changes through fission and fusion processes that regulate mitochondrial function, and it therefore plays a prominent role in cellular homeostasis. Cell death progression is associated with mitochondrial fission. Fission is mediated by the mainly cytoplasmic Drp1, which is activated by different post-translational modifications and recruited to mitochondria to perform its function. Our research and other studies have shown that in the early moments of excitotoxic insult Drp1 must be nitrosylated to mediate mitochondrial fragmentation in neurons. Nonetheless, mitochondrial fission is a multistep process in which filamentous actin assembly/disassembly and myosin-mediated mitochondrial constriction play prominent roles. Here we establish that in addition to nitric oxide production, excitotoxicity-induced mitochondrial fragmentation also requires activation of the actomyosin regulator ROCK. Although ROCK1 has been shown to phosphorylate and activate Drp1, experiments using phosphor-mutant forms of Drp1 in primary cortical neurons indicate that in excitotoxic conditions, ROCK does not act directly on Drp1 to mediate fission, but may act on the actomyosin complex. Thus, these data indicate that a wider range of signaling pathways than those that target Drp1 are amenable to be inhibited to prevent mitochondrial fragmentation as therapeutic option
Subject(s):	-Mitocondris -Patologia cel·lular -Mitochondria -Cellular pathology
Rights:	(c) Landes Bioscience , 2015
Document type:	Article Article - Accepted version
Published by:	Landes Bioscience
Share:

Show full item record