Título:
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Improving virus production through quasispecies genomic selection and molecular breedings
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Autor/a:
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Pérez Rodríguez, Francisco Javier, 1964-; D'Andrea, Lucia; Castellarnau Serra, Montserrat de; Costafreda Salvany, M. Isabel; Guix Arnau, Susana; Ribes Mora, Enric; Quer, Josep; Gregori Font, Josep; Bosch, Albert; Pintó Solé, Rosa María
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Otros autores:
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Universitat de Barcelona |
Abstract:
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Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential. |
Materia(s):
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-Virologia -Espècies (Biologia) -Virology -Species |
Derechos:
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cc-by (c) Pérez et al., 2016
http://creativecommons.org/licenses/by/3.0/es |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Nature Publishing Group
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Compartir:
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