Bacterial cellulose was combined with wood cellulose papers in order to obtain biomaterials with increased barrier properties. For this purpose, different parameters were assessed: two producing bacterial strains (Komagataeibacter xylinus and Gluconacetobacter sucrofermentans), two paper supports to hold bacterial cellulose (filter paper and eucalyptus paper), two kinds of combined biomaterials (composite and bilayer) and two drying temperatures (90 °C and room temperature). Papers with increased barrier properties (100° of water contact angle, 1220 s of water drop test and air permeability < 1 µm (Pa s)-1) were obtained by the addition of bacterial cellulose to each paper support. However, due to the lower initial barrier properties of filter paper, higher improvements were produced with this paper. In addition, bacterial cellulose provided smoother surfaces with higher gloss without a detrimental effect on physical properties. Higher resistance to water absorption was obtained with K. xylinus possibly explained by its longer size fibers than G. sucrofermentans, as analysed by SEM. Smoothness and gloss were specially increased in the bilayer biomaterial although resistance to air and water were further improved in the composite. In this biomaterial drying at high temperature had a detrimental effect. SEM analysis of the products obtained showed the intimate contact among fibers of bacterial cellulose and wood paper. Results obtained show the contribution of bacterial cellulose to improve the properties of paper and its potential for the design of new added value paper products from biomass