miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation

Home | About RECERCAT | Contact

Català | Castellano

All of RECERCAT

By Communities &
Collections By Defense Date By Authors By Titles By Subject

This Collection

By Defense Date By Authors By Titles By Subject

Statistics

View Statistics All RECERCAT

My RECERCAT

Other repositories directory

RECERCAT Home > Universitat de Barcelona > Ciències Clíniques > Articles publicats en revistes (Ciències Clíniques) > View document

To access the full text documents, please follow this link: http://hdl.handle.net/2445/119377

dc.contributor	Universitat de Barcelona
dc.contributor.author	Viñas Muñoz, José Luis
dc.contributor.author	Ventayol Espinazo, Marina
dc.contributor.author	Brüne, Bernhard
dc.contributor.author	Jung, Michaela
dc.contributor.author	Sola Martínez, Anna
dc.contributor.author	Pi i Siqués, Felip
dc.contributor.author	Mastora, Chrysoula
dc.contributor.author	Hotter Corripio, Georgina
dc.date	2018-01-29T12:38:35Z
dc.date	2018-01-29T12:38:35Z
dc.date	2013-04-10
dc.date	2018-01-29T12:38:35Z
dc.identifier.citation	1932-6203
dc.identifier.citation	621721
dc.identifier.uri	http://hdl.handle.net/2445/119377
dc.format	8 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Public Library of Science (PLoS)
dc.relation	Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0060937
dc.relation	PLoS One, 2013, vol. 8, num. 4, p. e60937
dc.relation	https://doi.org/10.1371/journal.pone.0060937
dc.rights	cc-by (c) Viñas Muñoz, José Luis et al., 2013
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.subject	Cèl·lules mare embrionàries
dc.subject	Micro RNAs
dc.subject	Diferenciació cel·lular
dc.subject	Embryonic stem cells
dc.subject	MicroRNAs
dc.subject	Cell diferentiation
dc.title	miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.abstract	This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.

Show simple item record

All of RECERCAT

This Collection

Statistics

My RECERCAT

Related documents

Other documents of the same author