dc.contributor.author |
Pons, Maria J. |
dc.contributor.author |
Gomes, Cláudia |
dc.contributor.author |
Aguilar, Ruth |
dc.contributor.author |
Barrios, Diana |
dc.contributor.author |
Aguilar-Luis, Miguel A. |
dc.contributor.author |
Ruiz, Joaquim |
dc.contributor.author |
Dobaño, Carlota, 1969- |
dc.contributor.author |
Valle Mendoza, Juana del |
dc.contributor.author |
Moncunill, Gemma |
dc.date |
2017-07-06T12:32:53Z |
dc.date |
2017-07-06T12:32:53Z |
dc.date |
2017-06-19 |
dc.date |
2017-06-21T18:01:03Z |
dc.identifier.citation |
1935-2727 |
dc.identifier.uri |
http://hdl.handle.net/2445/113447 |
dc.format |
16 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Public Library of Science |
dc.relation |
Reproducció del document publicat a:
http://dx.doi.org/10.1371/journal.pntd.0005684 |
dc.relation |
PLoS Neglected Tropical Diseases, 2017, vol. 11, num. 6, p.
e0005684 |
dc.relation |
http://dx.doi.org/10.1371/journal.pntd.0005684 |
dc.rights |
cc by (c) Pons et al., 2017 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Citoquines |
dc.subject |
Malalties bacterianes |
dc.subject |
Perú |
dc.subject |
Cytokines |
dc.subject |
Bacterial diseases |
dc.subject |
Peru |
dc.title |
Immunosuppressive and angiogenic cytokine profile associated
with Bartonella bacilliformis infection in post-outbreak and
endemic areas of Carrion's disease in Peru |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Analysis of immune responses in Bartonella bacilliformis
carriers are needed to understand acquisition of immunity to
Carrion's disease and may allow identifying biomarkers
associated with bacterial infection and disease phases. Serum
samples from 144 healthy subjects from 5 villages in the North
of Peru collected in 2014 were analyzed. Four villages had a
Carrion's disease outbreak in 2013, and the other is a
traditionally endemic area. Thirty cytokines, chemokines and
growth factors were determined in sera by fluorescent bead-based
quantitative suspension array technology, and analyzed in
relation to available data on bacteremia quantified by RT-PCR,
and IgM and IgG levels measured by ELISA against B.
bacilliformis lysates. The presence of bacteremia was associated
with low concentrations of HGF (p = 0.005), IL-15 (p = 0.002),
IL-6 (p = 0.05), IP-10 (p = 0.008), MIG (p = 0.03) and
MIP-1alpha (p = 0.03). In multi-marker analysis, the same and
further TH1-related and pro-inflammatory biomarkers were
inversely associated with infection, whereas angiogenic
chemokines and IL-10 were positively associated. Only EGF and
eotaxin showed a moderate positive correlation with bacteremia.
IgM seropositivity, which reflects a recent acute infection, was
associated with lower levels of eotaxin (p = 0.05), IL-6 (p =
0.001), and VEGF (p = 0.03). Only GM-CSF and IL-10
concentrations were positively associated with higher levels of
IgM (p = 0.01 and p = 0.007). Additionally, IgG seropositivity
and levels were associated with high levels of angiogenic
markers VEGF (p = 0.047) and eotaxin (p = 0.006), respectively.
Our findings suggest that B. bacilliformis infection causes
immunosuppression, led in part by overproduction of IL-10. This
immunosuppression probably contributes to the chronicity of
asymptomatic infections favoring B. bacilliformis persistence in
the host, allowing the subsequent transmission to the vector. In
addition, angiogenic markers associated with bacteremia and IgG
levels may be related to the induction of endothelial cell
proliferation in cutaneous lesions during chronic infections,
being possible candidate biomarkers of asymptomatic infections. |