Títol:
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Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes
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Autor/a:
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Calderón Domínguez, María; Sebastián Muñoz, David; Fucho Salvador, Raquel; Weber Blattes, Minéia; Mir Bonnín, Joan Francesc; García-Casarrubios, Ester; Obregón, Maria Jesús; Zorzano Olarte, Antonio; Valverde, Ángela M.; Serra i Cucurull, Dolors; Herrero Rodríguez, Laura
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Altres autors:
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Universitat de Barcelona |
Abstract:
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The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders. |
Matèries:
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-Carnitina palmitoïl-transferasa 1 -Teixit adipós -Obesitat -Carnitine palmitoyltransferase I -Adipose tissues -Obesity |
Drets:
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cc-by (c) Calderon-Dominguez, María et al., 2016
http://creativecommons.org/licenses/by/3.0/es |
Tipus de document:
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Article Article - Versió publicada |
Publicat per:
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Public Library of Science (PLoS)
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