Viral uncoating is directional: exit of the genomic RNA in a common cold virus starts with the poly-(A) tail at the 3′-end

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RECERCAT Home > Universitat de Barcelona > Enginyeria Química i Química Analítica > Articles publicats en revistes (Enginyeria Química i Química Analítica) > View document

To access the full text documents, please follow this link: http://hdl.handle.net/2445/98786

dc.contributor	Universitat de Barcelona
dc.contributor.author	Harutyunyan, Shushan
dc.contributor.author	Kumar, Mohit
dc.contributor.author	Sedivy, Arthur
dc.contributor.author	Subirats i Vila, Xavier
dc.contributor.author	Kowalski, Heinrich
dc.contributor.author	Köhler, Gottfried
dc.contributor.author	Blaas, Dieter
dc.date	2016-05-24T07:44:03Z
dc.date	2016-05-24T07:44:03Z
dc.date	2013-04-01
dc.date	2016-05-18T16:50:16Z
dc.identifier.citation	1553-7374
dc.identifier.citation	631108
dc.identifier.uri	http://hdl.handle.net/2445/98786
dc.description.abstract	Upon infection, many RNA viruses reorganize their capsid for release of the genome into the host cell cytosol for replication. Often, this process is triggered by receptor binding and/or by the acidic environment in endosomes. In the genus Enterovirus, which includes more than 150 human rhinovirus (HRV) serotypes causing the common cold, there is persuasive evidence that the viral RNA exits single-stranded through channels formed in the protein shell. We have determined the time-dependent emergence of the RNA ends from HRV2 on incubation of virions at 56°C using hybridization with specific oligonucleotides and detection by fluorescence correlation spectroscopy. We report that psoralen UV crosslinking prevents complete RNA release, allowing for identification of the sequences remaining inside the capsid. We also present the structure of uncoating intermediates in which parts of the RNA are condensed and take the form of a rod that is directed roughly towards a two-fold icosahedral axis, the presumed RNA exit point. Taken together, in contrast to schemes frequently depicted in textbooks and reviews, our findings demonstrate that exit of the RNA starts from the 3′-end. This suggests that packaging also occurs in an ordered manner resulting in the 3′-poly-(A) tail becoming located close to a position of pore formation during conversion of the virion into a subviral particle. This directional genome release may be common to many icosahedral non-enveloped single-stranded RNA viruses
dc.format	14 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Public Library of Science (PLoS)
dc.relation	Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.ppat.1003270
dc.relation	PLoS Pathogens, 2013, vol. 9, num. 4, p. e1003270
dc.relation	http://dx.doi.org/10.1371/journal.ppat.1003270
dc.rights	cc-by (c) Harutyunyan, Shushan et al., 2013
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.subject	Reovirus
dc.subject	RNA
dc.subject	Hibridació
dc.subject	Reoviruses
dc.subject	RNA
dc.subject	Hybridization
dc.title	Viral uncoating is directional: exit of the genomic RNA in a common cold virus starts with the poly-(A) tail at the 3′-end
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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