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Polypyrrole-Supported Membrane Proteins for Bio-Inspired Ion Channels
Pérez Madrigal, Maria del Mar; Valle Mendoza, Luis Javier del; Armelín Diggroc, Elaine Aparecida; Michaux, Catherine; Roussel, Guillaume; Perpete, Eric; Alemán Llansó, Carlos
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química; Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables.; Universitat Politècnica de Catalunya. IMEM - Innovació, Modelització i Enginyeria en (BIO) Materials
Biomedical platforms constructed by immobilizing membrane proteins in matrixes made of synthetic organic polymers is a challenge because the structure and function of these proteins are affected by environmental conditions. In this work, an operative composite that regulates the diffusion of alkali ions has been prepared by functionalizing a supporting matrix made of poly(N-methylpyrrole) (PNMPy) with a beta-barrel membrane protein (Omp2a) that forms channels and pores. The protein has been unequivocally identified in the composite, and its structure has been shown to remain unaltered. The PNMPy-Omp2a platform fulfills properties typically associated with functional bio-interfaces with biomedical applications (e.g., biocompatibility, biodegrabadility, and hydrophilicity). The functionality of the immobilized protein has been examined by studying the passive ion transport response in the presence of electrolytic solutions with Na+ and K+ concentrations close to those found in blood. Although the behavior of PNMPy and PNMPy-Omp2a is very similar for solutions with very low concentration, the resistance of the latter decreases drastically when the concentration of ions increases to similar to 100 mM. This reduction reflects an enhanced ion exchange between the biocomposite and the electrolytic medium, which is not observed in PNMPy, evidencing that PNMPy-Omp2a is particularly well suited to prepare bioinspired channels and smart biosensors.
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria química
Polymers in medicine
bioinspired channels
electroactive polymer
electrochemical impedance spectroscopy
ion exchange
functional bio-interfaces
electrochemical impedance spectroscopy
ray photoelectron-spectroscopy
conducting polymers
biomimetic membranes
copolymer
composites
poly(n-methylpyrrole)
derivatives
morphology
nanotubes
Polímers
Biopolímers
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/submittedVersion
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