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Novel peptide-based platform for the dual presentation of biologically active peptide motifs on biomaterials
Mas Moruno, Carlos; Fraioli, Roberta; Abericio, Fernando; Manero Planella, José María; Gil Mur, Francisco Javier
Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. BIBITE - Biomaterials, Biomecànica i Enginyeria de Teixits
Biofunctionalization of metallic materials with cell adhesive molecules derived from the extracellular matrix is a feasible approach to improve cell-material interactions and enhance the biointegration of implant materials (e.g., osseointegration of bone implants). However, classical biomimetic strategies may prove insufficient to elicit complex and multiple biological signals required in the processes of tissue regeneration. Thus, newer strategies are focusing on installing multifunctionality on biomaterials. In this work, we introduce a novel peptide-based divalent platform with the capacity to simultaneously present distinct bioactive peptide motifs in a chemically controlled fashion. As a proof of concept, the integrin-binding sequences RGD and PHSRN were selected and introduced in the platform. The biofunctionalization of titanium with this platform showed a positive trend towards increased numbers of cell attachment, and statistically higher values of spreading and proliferation of osteoblast-like cells compared to control noncoated samples. Moreover, it displayed statistically comparable or improved cell responses compared to samples coated with the single peptides or with an equimolar mixture of the two motifs. Osteoblast-like cells produced higher levels of alkaline phosphatase on surfaces functionalized with the platform than on control titanium; however, these values were not statistically significant. This study demonstrates that these peptidic structures are versatile tools to convey multiple biofunctionality to biomaterials in a chemically defined manner.
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria dels materials
Biomedical materials
Peptides
biofunctionalization
cell adhesive peptide
cell adhesion
peptide platform
titanium
RGD peptide
PHSRN peptide
CYCLIC RGD PEPTIDES
BIOCOMPATIBLE IMPLANT SURFACES
OSTEOBLAST-ADHESIVE PEPTIDES
ROUGH TITANIUM SURFACES
MEDIATED CELL-ADHESION
SER-ARG-ASN
HUMAN FIBRONECTIN
IN-VIVO
REGENERATIVE MEDICINE
INTEGRIN SPECIFICITY
Biomaterials
Pèptids
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
Article
         

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