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G protein-coupled receptor heterodimerization in the brain
Borroto Escuela, Dasiel Óscar; Romero Fernandez, Wilber; Garriga Solé, Pere; Ciruela, Francisco; Narvaez, Manuel; Tarakanov, O.V.; Palkovits, Miklós; Agnati, Luigi F.; Fuxe, Kjell
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química; Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
G protein-coupled receptors (GPCRs) play critical roles in cellular processes and signaling and have been shown to form heteromers with diverge biochemical and/or pharmacological activities that are different from those of the corresponding monomers or homomers. However, despite extensive experimental results supporting the formation of GPCR heteromers in heterologous systems, the existence of such receptor heterocomplexes in the brain remains largely unknown, mostly because of the lack of appropriate methodology. Herein, we describe the in situ proximity ligation assay procedure underlining its high selectivity and sensitivity to image GPCR heteromers with confocal microscopy in brain sections. We describe here how the assay is performed and discuss advantages and disadvantages of this method compared with other available techniques.
Àrees temàtiques de la UPC::Ciències de la salut::Medicina
G proteins--Receptors
Brain
Cell receptors
Allosteric modulation
Depression
Fibroblast growth factor receptor
Neuronal plasticity
Receptor heterocomplex
Serotonin receptors
Proteïnes G -- Receptors
Cervell
Receptors cel·lulars
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
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