dc.contributor.author
Aviñó Andrés, Anna
dc.contributor.author
Mazzini, Stefania
dc.contributor.author
Gargallo Gómez, Raimundo
dc.contributor.author
Eritja i Casadellà, Ramon
dc.date.issued
2020-03-03T11:14:22Z
dc.date.issued
2020-03-03T11:14:22Z
dc.date.issued
2016-02-05
dc.date.issued
2020-03-03T11:14:22Z
dc.identifier
https://hdl.handle.net/2445/151798
dc.description.abstract
Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
dc.format
application/pdf
dc.relation
Reproducció del document publicat a: https://doi.org/10.3390/ijms17020211
dc.relation
International Journal of Molecular Sciences, 2016, vol. 17, num. 2, p. 211
dc.relation
https://doi.org/10.3390/ijms17020211
dc.rights
cc-by (c) Aviñó, Anna et al., 2016
dc.rights
http://creativecommons.org/licenses/by/3.0/es
dc.rights
info:eu-repo/semantics/openAccess
dc.source
Articles publicats en revistes (Enginyeria Química i Química Analítica)
dc.subject
Oligonucleòtids
dc.subject
Oligonucleotides
dc.title
The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
