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dc.contributor.author | Tejero Villalba, Rut |
---|---|
dc.contributor.author | Navarro Ponz, Alfons |
dc.contributor.author | Campayo Guillaumes, Marc |
dc.contributor.author | Viñolas Segarra, Núria |
dc.contributor.author | Marrades Sicart, Ramon Ma. |
dc.contributor.author | Cordeiro Santanach, Anna |
dc.contributor.author | Ruíz Martínez, Marc |
dc.contributor.author | Santasusagna, Sandra |
dc.contributor.author | Molins López-Rodó, Laureano |
dc.contributor.author | Ramírez Ruz, J. (José) |
dc.contributor.author | Monzó Planella, Mariano |
dc.date | 2020-01-23T12:30:56Z |
dc.date | 2020-01-23T12:30:56Z |
dc.date | 2014-07-08 |
dc.date | 2020-01-23T12:30:57Z |
dc.identifier | 1932-6203 |
dc.identifier | 642876 |
dc.identifier.uri | http://hdl.handle.net/2445/148554 |
dc.description | Several treatments in non-small cell lung cancer (NSCLC) are histology-dependent, and the need for histology-related markers is increasing. MicroRNAs (miRNAs) are promising molecular markers in multiple cancers and show differences in expression depending on histological subtype. The miRNA family miR-200 has been associated with the regulation of epithelial-mesenchymal (EMT)/mesenchymal-epithelial transition (MET). EMT involves profound phenotypic changes that include the loss of cell-cell adhesion, the loss of cell polarity, and the acquisition of migratory and invasive properties that facilitates metastasis. A dual role for the miR-200 family in the prognosis of several tumors has been related to tumor cell origin. However, the prognostic role and function of miR-200 family in early-stage NSCLC adenocarcinoma and squamous cell carcinoma (SCC) have not been well established. Methods: miRNA expression was determined using TaqMan assays in 155 tumors from resected NSCLC patients. Functional studies were conducted in three NSCLC cell lines: H23, A-549 and HCC-44. Results: High miR-200c expression was associated with shorter overall survival (OS) in the entire cohort (p = 0.024). High miR-200c (p = 0.0004) and miR-141 (p = 0.009) expression correlated with shorter OS in adenocarcinoma - but not in SCC. In the multivariate analysis, a risk score based on miR-141 and miR-200c expression emerged as an independent prognostic factor for OS in the entire cohort (OR, 2.787; p = 0.033) and in adenocarcinoma patients (OR, 10.649; p = 0.002). Functional analyses showed that miR-200c, was related to mesenchymal-epithelial transition (MET) and affected cell migration and E-cadherin levels, while overexpression of miR-141 reduced KLF6 protein levels and produced an increase of secretion of VEGFA in vitro (H23, p = 0.04; A-549, p = 0.03; HCC-44, p = 0.02) and was associated with higher blood microvessel density in patient tumor samples (p<0.001). Conclusion: High miR-141 and miR-200c expression are associated with shorter OS in NSCLC patients with adenocarcinoma through MET and angiogenesis. |
dc.format | 9 p. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | Public Library of Science (PLoS) |
dc.relation | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0101899 |
dc.relation | PLoS One, 2014, vol. 9, num. 7, p. e101899 |
dc.relation | https://doi.org/10.1371/journal.pone.0101899 |
dc.rights | cc-by (c) Tejero Villalba, Rut et al., 2014 |
dc.rights | http://creativecommons.org/licenses/by/3.0/es |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Migració cel·lular |
dc.subject | Pronòstic mèdic |
dc.subject | Histologia |
dc.subject | Cell migration |
dc.subject | Prognosis |
dc.subject | Histology |
dc.title | miR-141 and miR-200c as markers of overall survival in early stage non-small cell lung cancer adenocarcinoma |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |