High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome

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RECERCAT Home > Universitat de Barcelona > IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer > Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) > View document

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dc.contributor.author	López Guimet, Júlia
dc.contributor.author	Andilla, Jordi
dc.contributor.author	Loza Álvarez, Pablo
dc.contributor.author	Egea Guri, Gustavo
dc.date	2018-11-28T14:14:40Z
dc.date	2018-11-28T14:14:40Z
dc.date	2017-05-04
dc.date	2018-11-28T14:14:40Z
dc.identifier	2045-2322
dc.identifier	676395
dc.identifier	28473723
dc.identifier.uri	http://hdl.handle.net/2445/126543
dc.description	In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples. We showed that internal elastic laminae and successive 2(nd) laminae are injured to a different extent in murine Marfan aortae; in particular, the density and size of fenestrae changed. Moreover, microstructural injuries were concentrated in the aortic proximal and convex anatomical regions. Other parameters such as the waviness and thickness of each lamina remained unaltered. In conclusion, the method reported here is a useful, unique tool for en-face laminae microstructure assessment that can obtain quantitative three-dimensional information about vascular tissue. The application of this method to murine Marfan aortae clearly shows that the microstructural damage in elastic laminae is not equal throughout the thickness of the tunica media and in the different anatomical regions of the ascending aorta.
dc.format	13 p.
dc.format	application/pdf
dc.language	eng
dc.publisher	Nature Publishing Group
dc.relation	Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-01620-8
dc.relation	Scientific Reports, 2017, vol. 7
dc.relation	https://doi.org/10.1038/s41598-017-01620-8
dc.relation	info:eu-repo/grantAgreement/EC/H2020/654148/EU//LASERLAB-EUROPE
dc.rights	cc-by (c) Lopez-Guimet, Julia et al., 2017
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Malalties hereditàries
dc.subject	Malalties del teixit connectiu
dc.subject	Aneurismes aòrtics
dc.subject	Genetic diseases
dc.subject	Connective tissues diseases
dc.subject	Aortic aneurysms
dc.title	High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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