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dc.contributor.author | Le Lay, Soazig |
---|---|
dc.contributor.author | Rodriguez, Macarena |
dc.contributor.author | Jessup, Wendy |
dc.contributor.author | Rentero Alfonso, Carles |
dc.contributor.author | Li, Qiong |
dc.contributor.author | Cartland, Siân |
dc.contributor.author | Grewal, Thomas |
dc.contributor.author | Gaus, Katharina |
dc.date | 2018-06-11T12:56:12Z |
dc.date | 2018-06-11T12:56:12Z |
dc.date | 2011-08-12 |
dc.date | 2018-06-11T12:56:12Z |
dc.identifier | 1932-6203 |
dc.identifier | 671620 |
dc.identifier | 21858084 |
dc.identifier.uri | http://hdl.handle.net/2445/122890 |
dc.description | Caveolin-1 (Cav1), a structural protein required for the formation of invaginated membrane domains known as caveolae, has been implicated in cholesterol trafficking and homeostasis. Here we investigated the contribution of Cav1 to apolipoprotein A-I (apoA-I) cell surface binding and intracellular processing using mouse embryonic fibroblasts (MEFs) derived from wild type (WT) or Cav1-deficient (Cav1−/−) animals. We found that cells expressing Cav1 have 2.6-fold more apoA-I binding sites than Cav1−/− cells although these additional binding sites are not associated with detergent-free lipid rafts. Further, Cav1-mediated binding targets apoA-I for internalization and degradation and these processes are not correlated to cholesterol efflux. Despite lower apoA-I binding, cholesterol efflux from Cav1−/− MEFs is 1.7-fold higher than from WT MEFs. Stimulation of ABCA1 expression with an LXR agonist enhances cholesterol efflux from both WT and Cav1−/− cells without increasing apoA-I surface binding or affecting apoA-I processing. Our results indicate that there are at least two independent lipid binding sites for apoA-I; Cav1-mediated apoA-I surface binding and uptake is not linked to cholesterol efflux, indicating that membrane domains other than caveolae regulate ABCA1-mediated cholesterol efflux. |
dc.format | 11 p. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | Public Library of Science (PLoS) |
dc.relation | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0023353 |
dc.relation | PLoS One, 2011, vol. 6, num. 8, p. e23353 |
dc.relation | https://doi.org/10.1371/journal.pone.0023353 |
dc.rights | cc-by (c) Le Lay, Soazig et al., 2011 |
dc.rights | http://creativecommons.org/licenses/by/3.0/es |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Colesterol |
dc.subject | Membranes cel·lulars |
dc.subject | Cholesterol |
dc.subject | Cell membranes |
dc.title | Caveolin-1-mediated apolipoprotein A-I membrane binding sites are not required for cholesterol efflux |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |