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dc.contributor.author | Callejas, Francisco de Borja |
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dc.contributor.author | Martínez Antón, Ma. Asunción |
dc.contributor.author | Alobid, Isam |
dc.contributor.author | Fuentes Prado, Mireya |
dc.contributor.author | Cortijo, Julio |
dc.contributor.author | Picado Vallés, César |
dc.contributor.author | Roca i Ferrer, Jordi |
dc.contributor.author | Mullol i Miret, Joaquim |
dc.date | 2018-05-07T11:23:10Z |
dc.date | 2018-05-07T11:23:10Z |
dc.date | 2014-06-19 |
dc.date | 2018-05-07T11:23:10Z |
dc.identifier | 1932-6203 |
dc.identifier | 644656 |
dc.identifier | 643724 |
dc.identifier | 24945146 |
dc.identifier.uri | http://hdl.handle.net/2445/122131 |
dc.description | BACKGROUND: Primary human airway epithelial cells cultured in an air-liquid interface (ALI) develop a well-differentiated epithelium. However, neither characterization of mucociliar differentiation overtime nor the inflammatory function of reconstituted nasal polyp (NP) epithelia have been described. OBJECTIVES: 1st) To develop and characterize the mucociliar differentiation overtime of human epithelial cells of chronic rhinosinusitis with nasal polyps (CRSwNP) in ALI culture system; 2nd) To corroborate that 3D in vitro model of NP reconstituted epithelium maintains, compared to control nasal mucosa (NM), an inflammatory function. METHODS: Epithelial cells were obtained from 9 NP and 7 control NM, and differentiated in ALI culture for 28 days. Mucociliary differentiation was characterized at different times (0, 7, 14, 21, and 28 days) using ultrastructure analysis by electron microscopy; ΔNp63 (basal stem/progenitor cell), β-tubulin IV (cilia), and MUC5AC (goblet cell) expression by immunocytochemistry; and mucous (MUC5AC, MUC5B) and serous (Lactoferrin) secretion by ELISA. Inflammatory function of ALI cultures (at days 0, 14, and 28) through cytokine (IL-8, IL-1β, IL-6, IL-10, TNF-α, and IL-12p70) and chemokine (RANTES, MIG, MCP-1, IP-10, eotaxin-1, and GM-CSF) production was analysed by CBA (Cytometric Bead Array). RESULTS: In both NP and control NM ALI cultures, pseudostratified epithelium with ciliated, mucus-secreting, and basal cells were observed by electron microscopy at days 14 and 28. Displaying epithelial cell re-differentation, β-tubulin IV and MUC5AC positive cells increased, while ΔNp63 positive cells decreased overtime. No significant differences were found overtime in MUC5AC, MUC5B, and lactoferrin secretions between both ALI cultures. IL-8 and GM-CSF were significantly increased in NP compared to control NM regenerated epithelia. CONCLUSION: Reconstituted epithelia from human NP epithelial cells cultured in ALI system provides a 3D in vitro model that could be useful both for studying the role of epithelium in CRSwNP while developing new therapeutic strategies, including cell therapy, for CRSwNP |
dc.format | 12 p. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | Public Library of Science (PLoS) |
dc.relation | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0100537 |
dc.relation | PLoS One, 2014, vol. 9, num. 6, p. e100537 |
dc.relation | https://doi.org/10.1371/journal.pone.0100537 |
dc.rights | cc-by (c) Callejas, Francisco de Borja et al., 2014 |
dc.rights | http://creativecommons.org/licenses/by/3.0/es |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Cèl·lules epitelials |
dc.subject | Pòlips (Patologia) |
dc.subject | Diferenciació cel·lular |
dc.subject | Citoquines |
dc.subject | Inflamació |
dc.subject | Malalties del nas |
dc.subject | Epithelial cells |
dc.subject | Polyps (Pathology) |
dc.subject | Cell diferentiation |
dc.subject | Cytokines |
dc.subject | Inflammation |
dc.subject | Nose diseases |
dc.title | Reconstituted human upper airway epithelium as 3-d in vitro model for nasal polyposis. |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |